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@ARTICLE{Eekhoff:15795,
      author       = {Eekhoff, A. and Bonakdar, N. and Alonso, J.L. and Hoffmann,
                      B. and Goldmann, W.H.},
      title        = {{G}lomerular podocytes: {A} study of mechanical properties
                      and mechano-chemical signaling},
      journal      = {Biochemical and biophysical research communications},
      volume       = {406},
      issn         = {0006-291X},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {PreJuSER-15795},
      pages        = {229 - 233},
      year         = {2011},
      note         = {We thank Drs. Ben Fabry, Rudolf Merkel, Gerold Diez, James
                      Smith, and Anna Klemm for helpful comments, Tim Feichtmeier
                      for the cyclic stretch experiments on human umbilical cord
                      fibroblasts, Andrea Zang for helping with OMTC, and Wolfgang
                      Rubner for building a new cell stretcher. This work was
                      supported by grants from Bayerisch-Franzosisches
                      Hochschulzentrum, Deutscher Akademischer Austausch Dienst,
                      Bavaria California Technology Center, and Deutsche
                      Forschungsgemeinschaft.},
      abstract     = {Kidney glomeruli function as filters, allowing the passage
                      of small solutes and waste products into the urinary tract,
                      while retaining essential proteins and macromolecules in the
                      blood stream. These structures are under constant mechanical
                      stress due to fluid pressure, driving filtration across the
                      barrier. We mechanically stimulated adherent wildtype
                      podocytes using the methods of magnetic tweezer and twisting
                      as well as cell stretching. Attaching collagen IV-coated or
                      poly-l-lysine-coated magnetic beads to cell receptors
                      allowed for the determination of cellular stiffness.
                      Angiotensin II-treated podocytes showed slightly higher
                      stiffness than untreated cells, the cell fluidity (i.e.
                      internal dynamics) remained similar, and showed an increase
                      with force. The bead detachment (a measure of the binding
                      strength) was higher in angiotensin II-treated compared to
                      untreated podocytes. Magnetic twisting confirmed that
                      angiotensin II treatment of podocytes increases and CDTA
                      treatment decreases cell stiffness. However, treatment with
                      both angiotensin II and CDTA increased the cell stiffness
                      only slightly compared to solely CDTA-treated cells.
                      Exposing podocytes to cyclic, uniaxial stretch showed an
                      earlier onset of ERK(1/2) phosphorylation compared to MEF
                      (control) cells. These results indicate that angiotensin II
                      might free intracellularly stored calcium and affects
                      actomyosin contraction, and that mechanical stimulation
                      influences cell signaling.},
      keywords     = {Angiotensin II: pharmacology / Angiotensin II: physiology /
                      Animals / Cell Adhesion / Cytoskeleton: physiology / Kidney
                      Glomerulus: cytology / Mechanical Processes /
                      Mechanotransduction, Cellular / Mice / Mitogen-Activated
                      Protein Kinase 1: metabolism / Mitogen-Activated Protein
                      Kinase 3: metabolism / Podocytes: drug effects / Podocytes:
                      physiology / Angiotensin II (NLM Chemicals) /
                      Mitogen-Activated Protein Kinase 1 (NLM Chemicals) /
                      Mitogen-Activated Protein Kinase 3 (NLM Chemicals) / J
                      (WoSType)},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung},
      pid          = {G:(DE-Juel1)FUEK505},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21315064},
      UT           = {WOS:000288616200013},
      doi          = {10.1016/j.bbrc.2011.02.022},
      url          = {https://juser.fz-juelich.de/record/15795},
}