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000015800 0247_ $$2pmid$$apmid:21355605
000015800 0247_ $$2DOI$$a10.1021/jp108280q
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000015800 037__ $$aPreJuSER-15800
000015800 041__ $$aeng
000015800 082__ $$a530
000015800 084__ $$2WoS$$aChemistry, Physical
000015800 1001_ $$0P:(DE-Juel1)VDB99503$$aLoritz, H.M.$$b0$$uFZJ
000015800 245__ $$aMechamical Strength of Specific Bonds Acting Isolated or in Pairs: A Case Study on Engineered Proteins
000015800 260__ $$aWashington, DC$$bSoc.$$c2011
000015800 300__ $$a2582 - 2592
000015800 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000015800 440_0 $$03694$$aJournal of Physical Chemistry B$$v115$$x1520-6106$$y11
000015800 500__ $$aRecord converted from VDB: 12.11.2012
000015800 520__ $$aThe dynamic strength of multiple specific bonds exposed to external mechanical force is of significant interest for the understanding of biological adhesion. Exploiting the well-established FLAG tag technology, we engineered model proteins exhibiting no, one, or two identical binding sites for a monoclonal antibody. Bonds between these engineered proteins and the antibody were studied with dynamic force spectroscopy. On single bonds between a FLAG-tag and the antibody, we observed two regimes corresponding to two different activated complexes, that is, two intermediate states along the reaction path for bond breakage. Dynamic force spectroscopy on double bonds showed the same two regimes. The actual yield forces of double bonds slightly exceeded those of single bonds. A simplified kinetic model with analytical solutions was developed and used to interpret the measured spectra.
000015800 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000015800 588__ $$aDataset connected to Web of Science, Pubmed
000015800 650_2 $$2MeSH$$aAlgorithms
000015800 650_2 $$2MeSH$$aAntibodies, Monoclonal: chemistry
000015800 650_2 $$2MeSH$$aAntibodies, Monoclonal: immunology
000015800 650_2 $$2MeSH$$aAntibody Affinity
000015800 650_2 $$2MeSH$$aBiomechanics
000015800 650_2 $$2MeSH$$aGreen Fluorescent Proteins: genetics
000015800 650_2 $$2MeSH$$aKinetics
000015800 650_2 $$2MeSH$$aModels, Chemical
000015800 650_2 $$2MeSH$$aPeptides: chemistry
000015800 650_2 $$2MeSH$$aPeptides: genetics
000015800 650_2 $$2MeSH$$aPeptides: immunology
000015800 650_2 $$2MeSH$$aProbability
000015800 650_2 $$2MeSH$$aProtein Binding
000015800 650_2 $$2MeSH$$aProtein Engineering
000015800 650_2 $$2MeSH$$aRecombinant Proteins: chemistry
000015800 650_2 $$2MeSH$$aRecombinant Proteins: genetics
000015800 650_2 $$2MeSH$$aRecombinant Proteins: immunology
000015800 650_2 $$2MeSH$$aSpectrum Analysis: methods
000015800 650_2 $$2MeSH$$aStatistical Distributions
000015800 650_7 $$00$$2NLM Chemicals$$aAntibodies, Monoclonal
000015800 650_7 $$00$$2NLM Chemicals$$aPeptides
000015800 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000015800 650_7 $$0147336-22-9$$2NLM Chemicals$$aGreen Fluorescent Proteins
000015800 650_7 $$098849-88-8$$2NLM Chemicals$$aFLAG peptide
000015800 650_7 $$2WoSType$$aJ
000015800 7001_ $$0P:(DE-Juel1)VDB8902$$aKirchgeßner, N.$$b1$$uFZJ
000015800 7001_ $$0P:(DE-Juel1)161241$$aBorn, S.$$b2$$uFZJ
000015800 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b3$$uFZJ
000015800 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b4$$uFZJ
000015800 773__ $$0PERI:(DE-600)2006039-7$$a10.1021/jp108280q$$gVol. 115, p. 2582 - 2592$$p2582 - 2592$$q115<2582 - 2592$$tThe @journal of physical chemistry <Washington, DC> / B$$v115$$x1520-6106$$y2011
000015800 8567_ $$uhttp://dx.doi.org/10.1021/jp108280q
000015800 909CO $$ooai:juser.fz-juelich.de:15800$$pVDB
000015800 9131_ $$0G:(DE-Juel1)FUEK505$$bSchlüsseltechnologien$$kP45$$lBiologische Informationsverarbeitung$$vBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$x0
000015800 9132_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft  Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000015800 9141_ $$y2011
000015800 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000015800 9201_ $$0I:(DE-Juel1)ICS-7-20110106$$gICS$$kICS-7$$lBiomechanik$$x0
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