TY  - JOUR
AU  - Loritz, H.M.
AU  - Kirchgeßner, N.
AU  - Born, S.
AU  - Hoffmann, B.
AU  - Merkel, R.
TI  - Mechamical Strength of Specific Bonds Acting Isolated or in Pairs: A Case Study on Engineered Proteins
JO  - The journal of physical chemistry  / B
VL  - 115
SN  - 1520-6106
CY  - Washington, DC
PB  - Soc.
M1  - PreJuSER-15800
SP  - 2582 - 2592
PY  - 2011
N1  - Record converted from VDB: 12.11.2012
AB  - The dynamic strength of multiple specific bonds exposed to external mechanical force is of significant interest for the understanding of biological adhesion. Exploiting the well-established FLAG tag technology, we engineered model proteins exhibiting no, one, or two identical binding sites for a monoclonal antibody. Bonds between these engineered proteins and the antibody were studied with dynamic force spectroscopy. On single bonds between a FLAG-tag and the antibody, we observed two regimes corresponding to two different activated complexes, that is, two intermediate states along the reaction path for bond breakage. Dynamic force spectroscopy on double bonds showed the same two regimes. The actual yield forces of double bonds slightly exceeded those of single bonds. A simplified kinetic model with analytical solutions was developed and used to interpret the measured spectra.
KW  - Algorithms
KW  - Antibodies, Monoclonal: chemistry
KW  - Antibodies, Monoclonal: immunology
KW  - Antibody Affinity
KW  - Biomechanics
KW  - Green Fluorescent Proteins: genetics
KW  - Kinetics
KW  - Models, Chemical
KW  - Peptides: chemistry
KW  - Peptides: genetics
KW  - Peptides: immunology
KW  - Probability
KW  - Protein Binding
KW  - Protein Engineering
KW  - Recombinant Proteins: chemistry
KW  - Recombinant Proteins: genetics
KW  - Recombinant Proteins: immunology
KW  - Spectrum Analysis: methods
KW  - Statistical Distributions
KW  - Antibodies, Monoclonal (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Green Fluorescent Proteins (NLM Chemicals)
KW  - FLAG peptide (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21355605
UR  - <Go to ISI:>//WOS:000288401100013
DO  - DOI:10.1021/jp108280q
UR  - https://juser.fz-juelich.de/record/15800
ER  -