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000016085 0247_ $$2pmc$$apmc:PMC3140484
000016085 0247_ $$2DOI$$a10.1371/journal.pone.0022143
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000016085 084__ $$2WoS$$aBiology
000016085 1001_ $$0P:(DE-Juel1)VDB78945$$aSchelder, S.$$b0$$uFZJ
000016085 245__ $$aThe two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress
000016085 260__ $$aLawrence, Kan.$$bPLoS$$c2011
000016085 29510 $$aonline available
000016085 300__ $$ae22143
000016085 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000016085 520__ $$aCopper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu(2+) was studied using DNA microarrays and revealed 20 genes that showed a ≥ 3-fold increased mRNA level, including cg3281-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. g. a multicopper oxidase (CopO) and a copper-transport ATPase (CopB). In addition, this region includes the copRS genes (previously named cgtRS9) which encode a two-component signal transduction system composed of the histidine kinase CopS and the response regulator CopR. Deletion of the copRS genes increased the sensitivity of C. glutamicum towards copper ions, but not to other heavy metal ions. Using comparative transcriptome analysis of the ΔcopRS mutant and the wild type in combination with electrophoretic mobility shift assays and reporter gene studies the CopR regulon and the DNA-binding motif of CopR were identified. Evidence was obtained that CopR binds only to the intergenic region between cg3285 (copR) and cg3286 in the genome of C. glutamicum and activates expression of the divergently oriented gene clusters cg3285-cg3281 and cg3286-cg3289. Altogether, our data suggest that CopRS is the key regulatory system in C. glutamicum for the extracytoplasmic sensing of elevated copper ion concentrations and for induction of a set of genes capable of diminishing copper stress.
000016085 536__ $$0G:(DE-Juel1)FUEK410$$2G:(DE-HGF)$$aBiotechnologie$$cPBT$$x0
000016085 588__ $$aDataset connected to Web of Science, Pubmed
000016085 650_2 $$2MeSH$$aAdaptation, Physiological: drug effects
000016085 650_2 $$2MeSH$$aAdaptation, Physiological: genetics
000016085 650_2 $$2MeSH$$aBacterial Proteins: genetics
000016085 650_2 $$2MeSH$$aBacterial Proteins: metabolism
000016085 650_2 $$2MeSH$$aBase Sequence
000016085 650_2 $$2MeSH$$aBinding Sites
000016085 650_2 $$2MeSH$$aCopper: toxicity
000016085 650_2 $$2MeSH$$aCorynebacterium glutamicum: cytology
000016085 650_2 $$2MeSH$$aCorynebacterium glutamicum: drug effects
000016085 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000016085 650_2 $$2MeSH$$aCorynebacterium glutamicum: physiology
000016085 650_2 $$2MeSH$$aDNA, Bacterial: genetics
000016085 650_2 $$2MeSH$$aDNA, Bacterial: metabolism
000016085 650_2 $$2MeSH$$aGene Expression Regulation, Bacterial: drug effects
000016085 650_2 $$2MeSH$$aGene Expression Regulation, Bacterial: genetics
000016085 650_2 $$2MeSH$$aGenes, Bacterial: genetics
000016085 650_2 $$2MeSH$$aHomeostasis: drug effects
000016085 650_2 $$2MeSH$$aHomeostasis: genetics
000016085 650_2 $$2MeSH$$aMutation
000016085 650_2 $$2MeSH$$aNucleotide Motifs: genetics
000016085 650_2 $$2MeSH$$aPhosphorylation: drug effects
000016085 650_2 $$2MeSH$$aPhosphorylation: genetics
000016085 650_2 $$2MeSH$$aProtein Kinases: genetics
000016085 650_2 $$2MeSH$$aProtein Kinases: metabolism
000016085 650_2 $$2MeSH$$aSignal Transduction: drug effects
000016085 650_2 $$2MeSH$$aSignal Transduction: genetics
000016085 650_2 $$2MeSH$$aStress, Physiological: drug effects
000016085 650_2 $$2MeSH$$aStress, Physiological: genetics
000016085 650_7 $$00$$2NLM Chemicals$$aBacterial Proteins
000016085 650_7 $$00$$2NLM Chemicals$$aDNA, Bacterial
000016085 650_7 $$07440-50-8$$2NLM Chemicals$$aCopper
000016085 650_7 $$0EC 2.7.-$$2NLM Chemicals$$aProtein Kinases
000016085 650_7 $$0EC 2.7.3.-$$2NLM Chemicals$$aprotein-histidine kinase
000016085 650_7 $$2WoSType$$aJ
000016085 7001_ $$0P:(DE-HGF)0$$aZaade, D.$$b1
000016085 7001_ $$0P:(DE-Juel1)VDB78944$$aLitsanov, B.$$b2$$uFZJ
000016085 7001_ $$0P:(DE-Juel1)128943$$aBott, M.$$b3$$uFZJ
000016085 7001_ $$0P:(DE-Juel1)VDB13864$$aBrocker, M.$$b4$$uFZJ
000016085 773__ $$0PERI:(DE-600)2267670-3$$a10.1371/journal.pone.0022143$$gVol. 6, p. e22143$$pe22143$$q6<e22143$$tPLoS one$$v6$$x1932-6203$$y2011
000016085 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140484
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000016085 9141_ $$y2011
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