TY  - JOUR
AU  - Schelder, S.
AU  - Zaade, D.
AU  - Litsanov, B.
AU  - Bott, M.
AU  - Brocker, M.
TI  - The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress
JO  - PLoS one
VL  - 6
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - PreJuSER-16085
SP  - e22143
PY  - 2011
N1  - Record converted from VDB: 12.11.2012
AB  - Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu(2+) was studied using DNA microarrays and revealed 20 genes that showed a ≥ 3-fold increased mRNA level, including cg3281-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. g. a multicopper oxidase (CopO) and a copper-transport ATPase (CopB). In addition, this region includes the copRS genes (previously named cgtRS9) which encode a two-component signal transduction system composed of the histidine kinase CopS and the response regulator CopR. Deletion of the copRS genes increased the sensitivity of C. glutamicum towards copper ions, but not to other heavy metal ions. Using comparative transcriptome analysis of the ΔcopRS mutant and the wild type in combination with electrophoretic mobility shift assays and reporter gene studies the CopR regulon and the DNA-binding motif of CopR were identified. Evidence was obtained that CopR binds only to the intergenic region between cg3285 (copR) and cg3286 in the genome of C. glutamicum and activates expression of the divergently oriented gene clusters cg3285-cg3281 and cg3286-cg3289. Altogether, our data suggest that CopRS is the key regulatory system in C. glutamicum for the extracytoplasmic sensing of elevated copper ion concentrations and for induction of a set of genes capable of diminishing copper stress.
KW  - Adaptation, Physiological: drug effects
KW  - Adaptation, Physiological: genetics
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: metabolism
KW  - Base Sequence
KW  - Binding Sites
KW  - Copper: toxicity
KW  - Corynebacterium glutamicum: cytology
KW  - Corynebacterium glutamicum: drug effects
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: physiology
KW  - DNA, Bacterial: genetics
KW  - DNA, Bacterial: metabolism
KW  - Gene Expression Regulation, Bacterial: drug effects
KW  - Gene Expression Regulation, Bacterial: genetics
KW  - Genes, Bacterial: genetics
KW  - Homeostasis: drug effects
KW  - Homeostasis: genetics
KW  - Mutation
KW  - Nucleotide Motifs: genetics
KW  - Phosphorylation: drug effects
KW  - Phosphorylation: genetics
KW  - Protein Kinases: genetics
KW  - Protein Kinases: metabolism
KW  - Signal Transduction: drug effects
KW  - Signal Transduction: genetics
KW  - Stress, Physiological: drug effects
KW  - Stress, Physiological: genetics
KW  - Bacterial Proteins (NLM Chemicals)
KW  - DNA, Bacterial (NLM Chemicals)
KW  - Copper (NLM Chemicals)
KW  - Protein Kinases (NLM Chemicals)
KW  - protein-histidine kinase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21799779
C2  - pmc:PMC3140484
UR  - <Go to ISI:>//WOS:000292931200027
DO  - DOI:10.1371/journal.pone.0022143
UR  - https://juser.fz-juelich.de/record/16085
ER  -