guest ::
| Home > Publications database > The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress > print |
| Home > Publications database > The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress > print |
| 001 | 16085 | ||
| 005 | 20200423203019.0 | ||
| 024 | 7 | _ | |a pmid:21799779 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC3140484 |2 pmc |
| 024 | 7 | _ | |a 10.1371/journal.pone.0022143 |2 DOI |
| 024 | 7 | _ | |a WOS:000292931200027 |2 WOS |
| 024 | 7 | _ | |a 2128/11178 |2 Handle |
| 037 | _ | _ | |a PreJuSER-16085 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 500 |
| 084 | _ | _ | |2 WoS |a Biology |
| 100 | 1 | _ | |a Schelder, S. |b 0 |u FZJ |0 P:(DE-Juel1)VDB78945 |
| 245 | _ | _ | |a The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress |
| 260 | _ | _ | |a Lawrence, Kan. |b PLoS |c 2011 |
| 295 | 1 | 0 | |a online available |
| 300 | _ | _ | |a e22143 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a PLOS One |x 1932-6203 |0 18181 |y 7 |v 6 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu(2+) was studied using DNA microarrays and revealed 20 genes that showed a ≥ 3-fold increased mRNA level, including cg3281-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. g. a multicopper oxidase (CopO) and a copper-transport ATPase (CopB). In addition, this region includes the copRS genes (previously named cgtRS9) which encode a two-component signal transduction system composed of the histidine kinase CopS and the response regulator CopR. Deletion of the copRS genes increased the sensitivity of C. glutamicum towards copper ions, but not to other heavy metal ions. Using comparative transcriptome analysis of the ΔcopRS mutant and the wild type in combination with electrophoretic mobility shift assays and reporter gene studies the CopR regulon and the DNA-binding motif of CopR were identified. Evidence was obtained that CopR binds only to the intergenic region between cg3285 (copR) and cg3286 in the genome of C. glutamicum and activates expression of the divergently oriented gene clusters cg3285-cg3281 and cg3286-cg3289. Altogether, our data suggest that CopRS is the key regulatory system in C. glutamicum for the extracytoplasmic sensing of elevated copper ion concentrations and for induction of a set of genes capable of diminishing copper stress. |
| 536 | _ | _ | |a Biotechnologie |c PBT |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK410 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Adaptation, Physiological: drug effects |
| 650 | _ | 2 | |2 MeSH |a Adaptation, Physiological: genetics |
| 650 | _ | 2 | |2 MeSH |a Bacterial Proteins: genetics |
| 650 | _ | 2 | |2 MeSH |a Bacterial Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Base Sequence |
| 650 | _ | 2 | |2 MeSH |a Binding Sites |
| 650 | _ | 2 | |2 MeSH |a Copper: toxicity |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: cytology |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: drug effects |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: genetics |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: physiology |
| 650 | _ | 2 | |2 MeSH |a DNA, Bacterial: genetics |
| 650 | _ | 2 | |2 MeSH |a DNA, Bacterial: metabolism |
| 650 | _ | 2 | |2 MeSH |a Gene Expression Regulation, Bacterial: drug effects |
| 650 | _ | 2 | |2 MeSH |a Gene Expression Regulation, Bacterial: genetics |
| 650 | _ | 2 | |2 MeSH |a Genes, Bacterial: genetics |
| 650 | _ | 2 | |2 MeSH |a Homeostasis: drug effects |
| 650 | _ | 2 | |2 MeSH |a Homeostasis: genetics |
| 650 | _ | 2 | |2 MeSH |a Mutation |
| 650 | _ | 2 | |2 MeSH |a Nucleotide Motifs: genetics |
| 650 | _ | 2 | |2 MeSH |a Phosphorylation: drug effects |
| 650 | _ | 2 | |2 MeSH |a Phosphorylation: genetics |
| 650 | _ | 2 | |2 MeSH |a Protein Kinases: genetics |
| 650 | _ | 2 | |2 MeSH |a Protein Kinases: metabolism |
| 650 | _ | 2 | |2 MeSH |a Signal Transduction: drug effects |
| 650 | _ | 2 | |2 MeSH |a Signal Transduction: genetics |
| 650 | _ | 2 | |2 MeSH |a Stress, Physiological: drug effects |
| 650 | _ | 2 | |2 MeSH |a Stress, Physiological: genetics |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Bacterial Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a DNA, Bacterial |
| 650 | _ | 7 | |0 7440-50-8 |2 NLM Chemicals |a Copper |
| 650 | _ | 7 | |0 EC 2.7.- |2 NLM Chemicals |a Protein Kinases |
| 650 | _ | 7 | |0 EC 2.7.3.- |2 NLM Chemicals |a protein-histidine kinase |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Zaade, D. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Litsanov, B. |b 2 |u FZJ |0 P:(DE-Juel1)VDB78944 |
| 700 | 1 | _ | |a Bott, M. |b 3 |u FZJ |0 P:(DE-Juel1)128943 |
| 700 | 1 | _ | |a Brocker, M. |b 4 |u FZJ |0 P:(DE-Juel1)VDB13864 |
| 773 | _ | _ | |a 10.1371/journal.pone.0022143 |g Vol. 6, p. e22143 |p e22143 |q 6 |t PLoS one |v 6 |y 2011 |x 1932-6203 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140484 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/16085/files/journal.pone.0022143.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/16085/files/journal.pone.0022143.gif?subformat=icon |x icon |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/16085/files/journal.pone.0022143.jpg?subformat=icon-180 |x icon-180 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/16085/files/journal.pone.0022143.jpg?subformat=icon-700 |x icon-700 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/16085/files/journal.pone.0022143.pdf?subformat=pdfa |x pdfa |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:16085 |p openaire |p open_access |p driver |p VDB |p dnbdelivery |
| 913 | 1 | _ | |k PBT |v Biotechnologie |l ohne FE |b außerhalb PoF |0 G:(DE-Juel1)FUEK410 |x 0 |
| 913 | 2 | _ | |a DE-HGF |b Key Technologies |l Key Technologies for the Bioeconomy |1 G:(DE-HGF)POF3-580 |0 G:(DE-HGF)POF3-581 |2 G:(DE-HGF)POF3-500 |v Biotechnology |x 0 |
| 914 | 1 | _ | |y 2011 |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 3.0 |0 LIC:(DE-HGF)CCBY3 |2 HGFVOC |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a JCR/ISI refereed |0 StatID:(DE-HGF)0010 |
| 920 | 1 | _ | |k IBT-1 |l Biotechnologie 1 |g IBT |z ab 31.10.10 weitergeführt als IBG-1 |0 I:(DE-Juel1)VDB55 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)129721 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | 1 | _ | |a FullTexts |
| 981 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|