TY  - JOUR
AU  - Alboresi, A.
AU  - Gerotto, D.
AU  - Cazzaniga, S.
AU  - Bassi, R.
AU  - Morosinotto, T.
TI  - A red-shifted antenna protein associated with Photosystem II in Physcomitrella patens
JO  - The journal of biological chemistry
VL  - 286
SN  - 0021-9258
CY  - Bethesda, Md.
PB  - Soc.
M1  - PreJuSER-16490
SP  - 28978 - 28987
PY  - 2011
N1  - This work was supported by the Cassa di Risparmio di Padova e Rovigo (CaRiPaRo) Foundation, Universita di Padova Grant CPDA089403, EEC Project Harvest and Fondo per gli Investimenti della Ricerca di Base-Parallelomics Grant RBIP06CTBR.
AB  - Antenna systems of plants and green algae are made up of pigment-protein complexes belonging to the light-harvesting complex (LHC) multigene family. LHCs increase the light-harvesting cross-section of photosystems I and II and catalyze photoprotective reactions that prevent light-induced damage in an oxygenic environment. The genome of the moss Physcomitrella patens contains two genes encoding LHCb9, a new antenna protein that bears an overall sequence similarity to photosystem II antenna proteins but carries a specific motif typical of photosystem I antenna proteins. This consists of the presence of an asparagine residue as a ligand for Chl 603 (A5) chromophore rather than a histidine, the common ligand in all other LHCbs. Asparagine as a Chl 603 (A5) ligand generates red-shifted spectral forms associated with photosystem I rather than with photosystem II, suggesting that in P. patens, the energy landscape of photosystem II might be different with respect to that of most green algae and plants. In this work, we show that the in vitro refolded LHCb9-pigment complexes carry a red-shifted fluorescence emission peak, different from all other known photosystem II antenna proteins. By using a specific antibody, we localized LHCb9 within PSII supercomplexes in the thylakoid membranes. This is the first report of red-shifted spectral forms in a PSII antenna system, suggesting that this biophysical feature might have a special role either in optimization of light use efficiency or in photoprotection in the specific environmental conditions experienced by this moss.
KW  - Bryopsida: chemistry
KW  - Bryopsida: genetics
KW  - Bryopsida: metabolism
KW  - Genome, Plant: physiology
KW  - Photosystem II Protein Complex: chemistry
KW  - Photosystem II Protein Complex: genetics
KW  - Photosystem II Protein Complex: metabolism
KW  - Plant Proteins: chemistry
KW  - Plant Proteins: genetics
KW  - Plant Proteins: metabolism
KW  - Photosystem II Protein Complex (NLM Chemicals)
KW  - Plant Proteins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21705318
C2  - pmc:PMC3190705
UR  - <Go to ISI:>//WOS:000293837000034
DO  - DOI:10.1074/jbc.M111.226126
UR  - https://juser.fz-juelich.de/record/16490
ER  -