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@ARTICLE{Becker:16495,
author = {Becker, J.Su. and Pozebon, D. and Matusch, A. and .
Dressler, V.L. and Becker, J.S.},
title = {{D}etection of {Z}n-containing proteins in slug ({G}enus
{A}rion) tissue using laser ablation {ICP}-{MS} after
separation by gel electrophoresis},
journal = {International journal of mass spectrometry},
volume = {307},
issn = {1387-3806},
address = {[S.l.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-16495},
year = {2011},
note = {Record converted from VDB: 12.11.2012},
abstract = {Assessing the inventory of biological systems in respect to
metal species is a growing area of life science research
called metallomics. Slugs are of special interest as monitor
organisms for environmental contaminations. Laser ablation
inductively coupled plasma mass spectrometry (LA-ICP-MS) was
applied to map the distribution of total Zn in a section of
a slug sample and to detect Zn-containing proteins after
one-dimensional separation by gel electrophoresis (Blue
Native PAGE). Interestingly, by far the largest fraction of
protein bound Zn was explained by three sharp and prominent
bands at 75,100 and 150 kDa. Analysis of tryptic digests of
selected bands using MALDI-TOF-MS and public databases
failed to identify proteins within the Zn bands what may be
due to coverage gaps concerning the species anon ater. Three
non-Zn containing bands could be assigned to proteins known
from other mollusc species. (C) 2011 Elsevier B.V. All
rights reserved.},
keywords = {J (WoSType)},
cin = {ZCH / INM-2},
ddc = {530},
cid = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)INM-2-20090406},
pnm = {Funktion und Dysfunktion des Nervensystems (FUEK409) /
89574 - Theory, modelling and simulation (POF2-89574)},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-HGF)POF2-89574},
shelfmark = {Physics, Atomic, Molecular $\&$ Chemical / Spectroscopy},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000295864100011},
doi = {10.1016/j.ijms.2011.02.015},
url = {https://juser.fz-juelich.de/record/16495},
}