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@ARTICLE{Matusch:16497,
author = {Matusch, A. and Bauer, A. and Becker, J.S.},
title = {{E}lement imaging in formalin fixed slices of human
mesencephalon},
journal = {International journal of mass spectrometry},
volume = {307},
issn = {1387-3806},
address = {[S.l.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-16497},
year = {2011},
note = {Record converted from VDB: 12.11.2012},
abstract = {The scarcity of native frozen pathological and healthy
human brain tissue is actually one of the major bottlenecks
concerning the investigation of neuropsychiatric diseases.
Therefore it is of some interest to make formalin fixed
tissue specimens accessible to mass spectrometric analytical
techniques. Laser ablation inductively coupled plasma mass
spectrometry (LA-ICP-MS) and matrix matched standards were
employed to generate quantitative element maps of human
mesencephalon slices that had been stored in formalin for
more than 10 years of a single subject. The characteristic
distribution patterns of Cu and Zn were completely blunted
but Fe was clearly enriched in the substantia nigra (SN) -
especially in the matrix of the pars compacta - and the
nucleus ruber (NR) at concentrations of 179 mu g g(-1), 231
mu g g(-1) and 187 mu g g(-1) (wet weight), respectively,
very similar to literature values obtained from homogenates
of fresh tissue. Mn and Ca congruently reflected the
distribution of melanised SN neurons. Interestingly, the
distribution of Pb was congruent to that of Fe reaching 0.8
mu g g(-1) in the SN, 1.1 mu g g(-1) in the matrix and 0.9
mu g g(-1) in the NR. Images of C and P reflected the gray
matter-white matter partition of the tissue. These results
suggest that a meaningful quantitative assessment of Fe, Mn
and Pb is possible in tissue samples which have previously
been stored in buffered formalin solution. (C) 2011 Elsevier
B.V. All rights reserved.},
keywords = {J (WoSType)},
cin = {ZCH / INM-2},
ddc = {530},
cid = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)INM-2-20090406},
pnm = {Funktion und Dysfunktion des Nervensystems (FUEK409) /
89574 - Theory, modelling and simulation (POF2-89574)},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-HGF)POF2-89574},
shelfmark = {Physics, Atomic, Molecular $\&$ Chemical / Spectroscopy},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000295864100033},
doi = {10.1016/j.ijms.2011.03.006},
url = {https://juser.fz-juelich.de/record/16497},
}