000016562 001__ 16562
000016562 005__ 20200423203029.0
000016562 0247_ $$2pmid$$apmid:21803939
000016562 0247_ $$2pmc$$apmc:PMC3226214
000016562 0247_ $$2DOI$$a10.1105/tpc.111.087320
000016562 0247_ $$2WOS$$aWOS:000294164300020
000016562 0247_ $$2ISSN$$a1040-4651
000016562 037__ $$aPreJuSER-16562
000016562 041__ $$aeng
000016562 082__ $$a570
000016562 084__ $$2WoS$$aBiochemistry & Molecular Biology
000016562 084__ $$2WoS$$aPlant Sciences
000016562 084__ $$2WoS$$aCell Biology
000016562 1001_ $$0P:(DE-HGF)0$$ade Bianchi, S.$$b0
000016562 245__ $$aArabidopsis Mutants Deleted in the Light-Harvesting Protein Lhcb4 Have a Disrupted Photosystem II Macrostructure and Are Defective in Photoprotection
000016562 260__ $$aRockville, Md.$$bSoc.$$c2011
000016562 300__ $$a2659 - 2679
000016562 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000016562 3367_ $$2DataCite$$aOutput Types/Journal article
000016562 3367_ $$00$$2EndNote$$aJournal Article
000016562 3367_ $$2BibTeX$$aARTICLE
000016562 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000016562 3367_ $$2DRIVER$$aarticle
000016562 440_0 $$022210$$aPlant Cell$$x1040-4651$$y23
000016562 500__ $$aWe thank Giovanni Finazzi (Commissariat a l'Energie Atomique, Grenoble, France) for helpful discussion. Financial support for this work was provided by the Programmi di Ricerca di Interesse Nazionale (2008XB774B) and by a grant of the Italian Ministry of Research and Marie Curie Actions Initial Training Networks HARVEST (Grant 238017).
000016562 520__ $$aThe role of the light-harvesting complex Lhcb4 (CP29) in photosynthesis was investigated in Arabidopsis thaliana by characterizing knockout lines for each of the three Lhcb4 isoforms (Lhcb4.1/4.2/4.3). Plants lacking all isoforms (koLhcb4) showed a compensatory increase of Lhcb1 and a slightly reduced photosystem II/I ratio with respect to the wild type. The absence of Lhcb4 did not result in alteration in electron transport rates. However, the kinetic of state transition was faster in the mutant, and nonphotochemical quenching activity was lower in koLhcb4 plants with respect to either wild type or mutants retaining a single Lhcb4 isoform. KoLhcb4 plants were more sensitive to photoinhibition, while this effect was not observed in knockout lines for any other photosystem II antenna subunit. Ultrastructural analysis of thylakoid grana membranes showed a lower density of photosystem II complexes in koLhcb4. Moreover, analysis of isolated supercomplexes showed a different overall shape of the C₂S₂ particles due to a different binding mode of the S-trimer to the core complex. An empty space was observed within the photosystem II supercomplex at the Lhcb4 position, implying that the missing Lhcb4 was not replaced by other Lhc subunits. This suggests that Lhcb4 is unique among photosystem II antenna proteins and determinant for photosystem II macro-organization and photoprotection.
000016562 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000016562 588__ $$aDataset connected to Web of Science, Pubmed
000016562 650_2 $$2MeSH$$aArabidopsis: genetics
000016562 650_2 $$2MeSH$$aArabidopsis: physiology
000016562 650_2 $$2MeSH$$aArabidopsis: ultrastructure
000016562 650_2 $$2MeSH$$aArabidopsis Proteins: genetics
000016562 650_2 $$2MeSH$$aArabidopsis Proteins: metabolism
000016562 650_2 $$2MeSH$$aChlorophyll: chemistry
000016562 650_2 $$2MeSH$$aChlorophyll Binding Proteins: genetics
000016562 650_2 $$2MeSH$$aChlorophyll Binding Proteins: metabolism
000016562 650_2 $$2MeSH$$aFluorescence
000016562 650_2 $$2MeSH$$aGene Knockdown Techniques
000016562 650_2 $$2MeSH$$aLight
000016562 650_2 $$2MeSH$$aLipid Peroxidation
000016562 650_2 $$2MeSH$$aMembrane Lipids: chemistry
000016562 650_2 $$2MeSH$$aMembrane Lipids: metabolism
000016562 650_2 $$2MeSH$$aOxidation-Reduction
000016562 650_2 $$2MeSH$$aOxidative Stress
000016562 650_2 $$2MeSH$$aOxygen: metabolism
000016562 650_2 $$2MeSH$$aPhotosynthesis: physiology
000016562 650_2 $$2MeSH$$aPhotosynthetic Reaction Center Complex Proteins: genetics
000016562 650_2 $$2MeSH$$aPhotosynthetic Reaction Center Complex Proteins: metabolism
000016562 650_2 $$2MeSH$$aPhotosystem I Protein Complex: metabolism
000016562 650_2 $$2MeSH$$aPhotosystem II Protein Complex: metabolism
000016562 650_2 $$2MeSH$$aPhotosystem II Protein Complex: ultrastructure
000016562 650_2 $$2MeSH$$aProtein Isoforms: genetics
000016562 650_2 $$2MeSH$$aProtein Isoforms: metabolism
000016562 650_2 $$2MeSH$$aTemperature
000016562 650_2 $$2MeSH$$aThylakoids: chemistry
000016562 650_2 $$2MeSH$$aThylakoids: metabolism
000016562 650_2 $$2MeSH$$aThylakoids: ultrastructure
000016562 650_7 $$00$$2NLM Chemicals$$aArabidopsis Proteins
000016562 650_7 $$00$$2NLM Chemicals$$aChlorophyll Binding Proteins
000016562 650_7 $$00$$2NLM Chemicals$$aLHCB4.1 protein, Arabidopsis
000016562 650_7 $$00$$2NLM Chemicals$$aMembrane Lipids
000016562 650_7 $$00$$2NLM Chemicals$$aPhotosynthetic Reaction Center Complex Proteins
000016562 650_7 $$00$$2NLM Chemicals$$aPhotosystem I Protein Complex
000016562 650_7 $$00$$2NLM Chemicals$$aPhotosystem II Protein Complex
000016562 650_7 $$00$$2NLM Chemicals$$aProtein Isoforms
000016562 650_7 $$01406-65-1$$2NLM Chemicals$$aChlorophyll
000016562 650_7 $$07782-44-7$$2NLM Chemicals$$aOxygen
000016562 650_7 $$2WoSType$$aJ
000016562 7001_ $$0P:(DE-HGF)0$$aBetterle, N.$$b1
000016562 7001_ $$0P:(DE-HGF)0$$aKouril, R.$$b2
000016562 7001_ $$0P:(DE-HGF)0$$aCazzaniga, S.$$b3
000016562 7001_ $$0P:(DE-HGF)0$$aBoekema, E.$$b4
000016562 7001_ $$0P:(DE-Juel1)VDB38860$$aBassi, R.$$b5$$uFZJ
000016562 7001_ $$0P:(DE-HGF)0$$aDall'Osto, L.$$b6
000016562 773__ $$0PERI:(DE-600)2004373-9$$a10.1105/tpc.111.087320$$gp. 2659 - 2679$$p2659 - 2679$$q2659 - 2679$$tThe Plant Cell$$x1040-4651$$y2011
000016562 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226214
000016562 8564_ $$uhttps://juser.fz-juelich.de/record/16562/files/FZJ-16562.pdf$$yRestricted$$zPublished final document.
000016562 909CO $$ooai:juser.fz-juelich.de:16562$$pVDB
000016562 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000016562 915__ $$0StatID:(DE-HGF)0020$$2StatID$$aNo Peer review
000016562 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000016562 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000016562 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000016562 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000016562 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000016562 9141_ $$y2011
000016562 9131_ $$0G:(DE-Juel1)FUEK407$$aDE-HGF$$bErde und Umwelt$$kP24$$lTerrestrische Umwelt$$vTerrestrische Umwelt$$x0
000016562 9132_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000016562 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$gIBG$$kIBG-2$$lPflanzenwissenschaften$$x0
000016562 970__ $$aVDB:(DE-Juel1)130702
000016562 980__ $$aVDB
000016562 980__ $$aConvertedRecord
000016562 980__ $$ajournal
000016562 980__ $$aI:(DE-Juel1)IBG-2-20101118
000016562 980__ $$aUNRESTRICTED