TY  - JOUR
AU  - Miloslavina, Y.
AU  - de Bianchi, S.
AU  - Dall'Osto, L.
AU  - Bassi, R.
AU  - Holzwarth, A.R.
TI  - Quenching in Arabidopsis thaliana Mutants Lacking Monomeric Antenna Proteins of Photosystem II
JO  - The journal of biological chemistry
VL  - 286
SN  - 0021-9258
CY  - Bethesda, Md.
PB  - Soc.
M1  - PreJuSER-16563
SP  - 36830 - 36840
PY  - 2011
N1  - This work was supported by the Marie Curie Initial Training Network "HARVEST" Grant 238017 within the FP7 program of the European Union, Sonderforschungsbereich Grant SFB 663, Heinrich-Heine-Universitat Dusseldorf, and the Max-Planck-Institutes Mulheim a.d. Ruhr.
AB  - The minor light-harvesting complexes CP24, CP26, and CP29 have been proposed to play a key role in the zeaxanthin (Zx)-dependent high light-induced regulation (NPQ) of excitation energy in higher plants. To characterize the detailed roles of these minor complexes in NPQ and to determine their specific quenching effects we have studied the ultrafast fluorescence kinetics in knockout (ko) mutants koCP26, koCP29, and the double mutant koCP24/CP26. The data provide detailed insight into the quenching processes and the reorganization of the Photosystem (PS) II supercomplex under quenching conditions. All genotypes showed two NPQ quenching sites. Quenching site Q1 is formed by a light-induced functional detachment of parts of the PSII supercomplex and a pronounced quenching of the detached antenna parts. The antenna remaining bound to the PSII core was also quenched substantially in all genotypes under NPQ conditions (quenching site Q2) as compared with the dark-adapted state. The latter quenching was about equally strong in koCP26 and the koCP24/CP26 mutants as in the WT. Q2 quenching was substantially reduced, however, in koCP29 mutants suggesting a key role for CP29 in the total NPQ. The observed quenching effects in the knockout mutants are complicated by the fact that other minor antenna complexes do compensate in part for the lack of the CP24 and/or CP29 complexes. Their lack also causes some LHCII dissociation already in the dark.
KW  - Arabidopsis: genetics
KW  - Arabidopsis: metabolism
KW  - Arabidopsis Proteins: genetics
KW  - Arabidopsis Proteins: metabolism
KW  - Chloroplast Proteins: genetics
KW  - Chloroplast Proteins: metabolism
KW  - Gene Knockdown Techniques
KW  - Photosynthesis: physiology
KW  - Photosystem II Protein Complex: genetics
KW  - Photosystem II Protein Complex: metabolism
KW  - Plants, Genetically Modified: genetics
KW  - Plants, Genetically Modified: metabolism
KW  - Arabidopsis Proteins (NLM Chemicals)
KW  - Chloroplast Proteins (NLM Chemicals)
KW  - Photosystem II Protein Complex (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21844190
C2  - pmc:PMC3196121
UR  - <Go to ISI:>//WOS:000296538300063
DO  - DOI:10.1074/jbc.M111.273227
UR  - https://juser.fz-juelich.de/record/16563
ER  -