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@ARTICLE{Miloslavina:16563,
author = {Miloslavina, Y. and de Bianchi, S. and Dall'Osto, L. and
Bassi, R. and Holzwarth, A.R.},
title = {{Q}uenching in {A}rabidopsis thaliana {M}utants {L}acking
{M}onomeric {A}ntenna {P}roteins of {P}hotosystem {II}},
journal = {The journal of biological chemistry},
volume = {286},
issn = {0021-9258},
address = {Bethesda, Md.},
publisher = {Soc.},
reportid = {PreJuSER-16563},
pages = {36830 - 36840},
year = {2011},
note = {This work was supported by the Marie Curie Initial Training
Network "HARVEST" Grant 238017 within the FP7 program of the
European Union, Sonderforschungsbereich Grant SFB 663,
Heinrich-Heine-Universitat Dusseldorf, and the
Max-Planck-Institutes Mulheim a.d. Ruhr.},
abstract = {The minor light-harvesting complexes CP24, CP26, and CP29
have been proposed to play a key role in the zeaxanthin
(Zx)-dependent high light-induced regulation (NPQ) of
excitation energy in higher plants. To characterize the
detailed roles of these minor complexes in NPQ and to
determine their specific quenching effects we have studied
the ultrafast fluorescence kinetics in knockout (ko) mutants
koCP26, koCP29, and the double mutant koCP24/CP26. The data
provide detailed insight into the quenching processes and
the reorganization of the Photosystem (PS) II supercomplex
under quenching conditions. All genotypes showed two NPQ
quenching sites. Quenching site Q1 is formed by a
light-induced functional detachment of parts of the PSII
supercomplex and a pronounced quenching of the detached
antenna parts. The antenna remaining bound to the PSII core
was also quenched substantially in all genotypes under NPQ
conditions (quenching site Q2) as compared with the
dark-adapted state. The latter quenching was about equally
strong in koCP26 and the koCP24/CP26 mutants as in the WT.
Q2 quenching was substantially reduced, however, in koCP29
mutants suggesting a key role for CP29 in the total NPQ. The
observed quenching effects in the knockout mutants are
complicated by the fact that other minor antenna complexes
do compensate in part for the lack of the CP24 and/or CP29
complexes. Their lack also causes some LHCII dissociation
already in the dark.},
keywords = {Arabidopsis: genetics / Arabidopsis: metabolism /
Arabidopsis Proteins: genetics / Arabidopsis Proteins:
metabolism / Chloroplast Proteins: genetics / Chloroplast
Proteins: metabolism / Gene Knockdown Techniques /
Photosynthesis: physiology / Photosystem II Protein Complex:
genetics / Photosystem II Protein Complex: metabolism /
Plants, Genetically Modified: genetics / Plants, Genetically
Modified: metabolism / Arabidopsis Proteins (NLM Chemicals)
/ Chloroplast Proteins (NLM Chemicals) / Photosystem II
Protein Complex (NLM Chemicals) / J (WoSType)},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt / HARVEST - Control of Light Use
Efficiency in Plants and Algae - From Light to Harvest
(238017)},
pid = {G:(DE-Juel1)FUEK407 / G:(EU-Grant)238017},
shelfmark = {Biochemistry $\&$ Molecular Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21844190},
pmc = {pmc:PMC3196121},
UT = {WOS:000296538300063},
doi = {10.1074/jbc.M111.273227},
url = {https://juser.fz-juelich.de/record/16563},
}