TY  - JOUR
AU  - Schwarz, C.K.W.
AU  - Tschapek, B.
AU  - Jumpertz, T.
AU  - Jenewein, S.
AU  - Lecher, J.
AU  - Willbold, D.
AU  - Panjikar, S.
AU  - Halland, B.
AU  - Smits, S.H.J.
AU  - Schmitt, L.
TI  - Crystallisation and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter Haemolysin B
JO  - Acta crystallographica / F
VL  - 67
SN  - 1744-3091
CY  - Oxford [u.a.]
PB  - Blackwell
M1  - PreJuSER-16637
SP  - 630 - 633
PY  - 2011
N1  - We would like to acknowledge Solvej Siedler for important assistance during the initial stages of this project. This work was funded by the EDICT (European Initiative on Channels and Transporters) consortium FP7 Theme (Health-2007-2.1.1-5) to BT and LS. We thank the Ministry of Innovation, Science and Research of the German Federal State North Rhine-Westphalia (NRW) and the Heinrich Heine University Dusseldorf (scholarship from the CLIB-Graduate Cluster Industrial Biotechnology to CKWS).
AB  - The ABC transporter haemolysin B (HlyB) from Escherichia coli is part of a type I secretion system that translocates a 110 kDa toxin in one step across both membranes of this Gram-negative bacterium in an ATP-dependent manner. Sequence analysis indicates that HlyB contains a C39 peptidase-like domain at its N-terminus. C39 domains are thiol-dependent peptidases that cleave their substrates after a GG motif. Interestingly, the catalytically invariant cysteine is replaced by a tyrosine in the C39-like domain of HlyB. Here, the overexpression, purification and crystallization of the isolated C39-like domain are described as a first step towards obtaining structural insights into this domain and eventually answering the question concerning the function of a degenerated C39 domain in the ABC transporter HlyB.
KW  - ATP-Binding Cassette Transporters: chemistry
KW  - Bacterial Proteins: chemistry
KW  - Carrier Proteins: chemistry
KW  - Crystallization
KW  - Crystallography, X-Ray
KW  - Escherichia coli: chemistry
KW  - Hemolysin Proteins: chemistry
KW  - Protein Multimerization
KW  - Protein Refolding
KW  - ATP-Binding Cassette Transporters (NLM Chemicals)
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Carrier Proteins (NLM Chemicals)
KW  - Hemolysin Proteins (NLM Chemicals)
KW  - Hlyb protein, Bacteria (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21543878
C2  - pmc:PMC3087657
UR  - <Go to ISI:>//WOS:000290235900025
DO  - DOI:10.1107/S1744309111010876
UR  - https://juser.fz-juelich.de/record/16637
ER  -