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000016686 0247_ $$2pmid$$apmid:21174398
000016686 0247_ $$2DOI$$a10.1021/ja108626w
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000016686 041__ $$aeng
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000016686 084__ $$2WoS$$aChemistry, Multidisciplinary
000016686 1001_ $$0P:(DE-Juel1)VDB101259$$aSabir, T.$$b0$$uFZJ
000016686 245__ $$aGlobal Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
000016686 260__ $$aWashington, DC$$bAmerican Chemical Society$$c2011
000016686 300__ $$a1188 - 1191
000016686 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000016686 440_0 $$08502$$aJournal of the American Chemical Society$$v133$$x0002-7863$$y5
000016686 500__ $$3POF3_Assignment on 2016-02-29
000016686 500__ $$aWe thank the BBSRC (BB/G00269X/1) for support and Claus Seidel, Ralf Kuhnemuth, Suren Felekyan, Stefan Marawske, Alessandro Valeri, and Evangelos Sisamakis for assistance in developing our MFD setup. We thank Richard Henchman for helpful discussions. S.W.M. acknowledges the award of an EPSRC Advanced Research Fellowship (EP/D073154).
000016686 520__ $$aBranched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.
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000016686 588__ $$aDataset connected to Web of Science, Pubmed
000016686 650_2 $$2MeSH$$aBase Sequence
000016686 650_2 $$2MeSH$$aDNA: chemistry
000016686 650_2 $$2MeSH$$aFluorescence Resonance Energy Transfer
000016686 650_2 $$2MeSH$$aMolecular Dynamics Simulation
000016686 650_2 $$2MeSH$$aNucleic Acid Conformation
000016686 650_2 $$2MeSH$$aSolutions
000016686 650_7 $$00$$2NLM Chemicals$$aSolutions
000016686 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
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000016686 7001_ $$0P:(DE-Juel1)132018$$aSchröder, G.F.$$b1$$uFZJ
000016686 7001_ $$0P:(DE-Juel1)VDB101260$$aToulmin, A.$$b2$$uFZJ
000016686 7001_ $$0P:(DE-Juel1)VDB101261$$aMcGlynn, P.$$b3$$uFZJ
000016686 7001_ $$0P:(DE-Juel1)VDB101262$$aMagennis, S.W.$$b4$$uFZJ
000016686 773__ $$0PERI:(DE-600)1472210-0$$a10.1021/ja108626w$$gVol. 133, p. 1188 - 1191$$p1188 - 1191$$q133<1188 - 1191$$tJournal of the American Chemical Society$$v133$$x0002-7863$$y2011
000016686 8567_ $$uhttp://dx.doi.org/10.1021/ja108626w
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000016686 9132_ $$0G:(DE-HGF)POF3-559H$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vAddenda$$x0
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