TY  - JOUR
AU  - Sabir, T.
AU  - Schröder, G.F.
AU  - Toulmin, A.
AU  - McGlynn, P.
AU  - Magennis, S.W.
TI  - Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
JO  - Journal of the American Chemical Society
VL  - 133
SN  - 0002-7863
CY  - Washington, DC
PB  - American Chemical Society
M1  - PreJuSER-16686
SP  - 1188 - 1191
PY  - 2011
N1  - We thank the BBSRC (BB/G00269X/1) for support and Claus Seidel, Ralf Kuhnemuth, Suren Felekyan, Stefan Marawske, Alessandro Valeri, and Evangelos Sisamakis for assistance in developing our MFD setup. We thank Richard Henchman for helpful discussions. S.W.M. acknowledges the award of an EPSRC Advanced Research Fellowship (EP/D073154).
AB  - Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.
KW  - Base Sequence
KW  - DNA: chemistry
KW  - Fluorescence Resonance Energy Transfer
KW  - Molecular Dynamics Simulation
KW  - Nucleic Acid Conformation
KW  - Solutions
KW  - Solutions (NLM Chemicals)
KW  - DNA (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21174398
UR  - <Go to ISI:>//WOS:000287228500013
DO  - DOI:10.1021/ja108626w
UR  - https://juser.fz-juelich.de/record/16686
ER  -