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@ARTICLE{Sabir:16686,
author = {Sabir, T. and Schröder, G.F. and Toulmin, A. and McGlynn,
P. and Magennis, S.W.},
title = {{G}lobal {S}tructure of {F}orked {DNA} in {S}olution
{R}evealed by {H}igh-{R}esolution {S}ingle-{M}olecule
{FRET}},
journal = {Journal of the American Chemical Society},
volume = {133},
issn = {0002-7863},
address = {Washington, DC},
publisher = {American Chemical Society},
reportid = {PreJuSER-16686},
pages = {1188 - 1191},
year = {2011},
note = {We thank the BBSRC (BB/G00269X/1) for support and Claus
Seidel, Ralf Kuhnemuth, Suren Felekyan, Stefan Marawske,
Alessandro Valeri, and Evangelos Sisamakis for assistance in
developing our MFD setup. We thank Richard Henchman for
helpful discussions. S.W.M. acknowledges the award of an
EPSRC Advanced Research Fellowship (EP/D073154).},
abstract = {Branched DNA structures play critical roles in DNA
replication, repair, and recombination in addition to being
key building blocks for DNA nanotechnology. Here we combine
single-molecule multiparameter fluorescence detection and
molecular dynamics simulations to give a general approach to
global structure determination of branched DNA in solution.
We reveal an open, planar structure of a forked DNA molecule
with three duplex arms and demonstrate an ion-induced
conformational change. This structure will serve as a
benchmark for DNA-protein interaction studies.},
keywords = {Base Sequence / DNA: chemistry / Fluorescence Resonance
Energy Transfer / Molecular Dynamics Simulation / Nucleic
Acid Conformation / Solutions / Solutions (NLM Chemicals) /
DNA (NLM Chemicals) / J (WoSType)},
cin = {ICS-6},
ddc = {540},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {Funktion und Dysfunktion des Nervensystems / BioSoft:
Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-Juel1)FUEK505},
shelfmark = {Chemistry, Multidisciplinary},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21174398},
UT = {WOS:000287228500013},
doi = {10.1021/ja108626w},
url = {https://juser.fz-juelich.de/record/16686},
}