Home > Publications database > Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET > print

001     16686
005     20200402210045.0
024 7 _ |2 pmid
|a pmid:21174398
024 7 _ |2 DOI
|a 10.1021/ja108626w
024 7 _ |2 WOS
|a WOS:000287228500013
037 _ _ |a PreJuSER-16686
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Chemistry, Multidisciplinary
100 1 _ |0 P:(DE-Juel1)VDB101259
|a Sabir, T.
|b 0
|u FZJ
245 _ _ |a Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
260 _ _ |a Washington, DC
|b American Chemical Society
|c 2011
300 _ _ |a 1188 - 1191
336 7 _ |a Journal Article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 8502
|a Journal of the American Chemical Society
|v 133
|x 0002-7863
|y 5
500 _ _ |3 POF3_Assignment on 2016-02-29
500 _ _ |a We thank the BBSRC (BB/G00269X/1) for support and Claus Seidel, Ralf Kuhnemuth, Suren Felekyan, Stefan Marawske, Alessandro Valeri, and Evangelos Sisamakis for assistance in developing our MFD setup. We thank Richard Henchman for helpful discussions. S.W.M. acknowledges the award of an EPSRC Advanced Research Fellowship (EP/D073154).
520 _ _ |a Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.
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|a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Base Sequence
650 _ 2 |2 MeSH
|a DNA: chemistry
650 _ 2 |2 MeSH
|a Fluorescence Resonance Energy Transfer
650 _ 2 |2 MeSH
|a Molecular Dynamics Simulation
650 _ 2 |2 MeSH
|a Nucleic Acid Conformation
650 _ 2 |2 MeSH
|a Solutions
650 _ 7 |0 0
|2 NLM Chemicals
|a Solutions
650 _ 7 |0 9007-49-2
|2 NLM Chemicals
|a DNA
650 _ 7 |2 WoSType
|a J
700 1 _ |0 P:(DE-Juel1)132018
|a Schröder, G.F.
|b 1
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB101260
|a Toulmin, A.
|b 2
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB101261
|a McGlynn, P.
|b 3
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB101262
|a Magennis, S.W.
|b 4
|u FZJ
773 _ _ |0 PERI:(DE-600)1472210-0
|a 10.1021/ja108626w
|g Vol. 133, p. 1188 - 1191
|p 1188 - 1191
|q 133<1188 - 1191
|t Journal of the American Chemical Society
|v 133
|x 0002-7863
|y 2011
856 7 _ |u http://dx.doi.org/10.1021/ja108626w
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914 1 _ |y 2011
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