000017113 001__ 17113
000017113 005__ 20200812104055.0
000017113 0247_ $$2ISSN$$a1866-1793
000017113 020__ $$a978-3-89336-724
000017113 037__ $$aPreJuSER-17113
000017113 041__ $$aEnglish
000017113 1001_ $$0P:(DE-Juel1)VDB89160$$aNing, Jing$$b0$$eCorresponding author$$uFZJ
000017113 245__ $$aApplication of functional gene arrays for monitoring influences of plant/seasons on bacterial functions and community structures in constructed wetland (Bitterfeld, Germany)
000017113 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2011
000017113 300__ $$aXIV, 157 S.
000017113 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis
000017113 3367_ $$0PUB:(DE-HGF)3$$2PUB:(DE-HGF)$$aBook
000017113 3367_ $$02$$2EndNote$$aThesis
000017113 3367_ $$2DRIVER$$adoctoralThesis
000017113 3367_ $$2BibTeX$$aPHDTHESIS
000017113 3367_ $$2DataCite$$aOutput Types/Dissertation
000017113 3367_ $$2ORCID$$aDISSERTATION
000017113 4900_ $$0PERI:(DE-600)2445288-9$$aSchriften des Forschungszentrums Jülich : Energie & Umwelt / Energy & Environment$$v114$$x1866-1793
000017113 502__ $$aRWTH Aachen, Diss., 2011$$bDr. (Univ.)$$cRWTH Aachen$$d2011
000017113 500__ $$3POF3_Assignment on 2016-02-29
000017113 500__ $$aRecord converted from VDB: 12.11.2012
000017113 520__ $$aWith the increasing application of constructed wetlands (CWs) around the world to improve water quality, it becomes more and more important to gain details about microbial ecology in this “black box”. In order to better understand general relationships among bacterial community structures, functions and environmental influencing factors in CWs, soil samples taken from the planted and the unplanted CWs in Bitterfeld (Germany), as well as those taken in warm- and cold-seasons, were compared in this study by using functional gene arrays (the GeoChip). In addition, other assessment methods, such as chemical analyses, fluorescent microscopic enumeration and PCRbased DGGE ($\underline{D}$enaturing $\underline{G}$radient $\underline{G}$el $\underline{E}$lectrophoresis) were also applied to complete the conclusions obtained by using the GeoChip. In general, this study consisted of two parts. The first part focused on evaluations of suitable DNA preparation procedures for functional gene arrays. The whole genome amplification was taken out of the primary plan due to biases in proportions of bacterial populations in DGGE band patterns, which were observed not only among the replications of the same DNA template but also between the products with and without this treatment. While evaluating procedure of DNA purification, the gel filtration column failed to purify DNA extracts of our samples, probably due to high amount of humic substances in the extracts. Among the three DNA extraction kits compared in this study (e.g. Bio101 FastDNA Spin Kit for Soil, UltraClean Soil DNA Isolation Kit and PowerSoil DNA Isolation Kit), extracts of the Bio101 were proved to be more suitable for the DGGE analysis with respect to their purity, yield and representativeness, while those of the PowerSoil showed better results in the microarray analysis. In addition, basing on the DNA extracts revealing different quantity and purity indices (A260/280, A260/230 and A320), influences of these indices on subsequent assessment methods of microbial communities (e.g. PCR-based DGGE and functional gene arrays) were also investigated. It was concluded that the success in PCR performance and consequently also in the DGGE analysis is more affected by the A260/280 and A320 values than by the ratio A260/230, and conditionally by the DNA yield. Moreover, the DGGE band pattern could also be affected by the preferential extraction due to chemical agents applied in the extraction. However, unlike the DGGE analysis, DNA microarrays were more affected by the A260/230 and A320 values. Thus, the primary goal of DNA extraction to obtain DNA extracts with the highest purity is not accurate enough any more in the environmental studies applying various analyzing methods. The performance of DNA extraction should vary with the subsequent [...]
000017113 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000017113 655_7 $$aHochschulschrift$$xDissertation (Univ.)
000017113 8564_ $$uhttps://juser.fz-juelich.de/record/17113/files/FZJ-17113.pdf$$yRestricted$$zPrepress version for printing
000017113 909CO $$ooai:juser.fz-juelich.de:17113$$pVDB:Earth_Environment$$pVDB
000017113 9141_ $$y2011
000017113 9131_ $$0G:(DE-Juel1)FUEK407$$bErde und Umwelt$$kP24$$lTerrestrische Umwelt$$vTerrestrische Umwelt$$x0
000017113 9132_ $$0G:(DE-HGF)POF3-259H$$1G:(DE-HGF)POF3-250$$2G:(DE-HGF)POF3-200$$aDE-HGF$$bMarine, Küsten- und Polare Systeme$$lTerrestrische Umwelt$$vAddenda$$x0
000017113 920__ $$lyes
000017113 9201_ $$0I:(DE-Juel1)IBG-3-20101118$$gIBG$$kIBG-3$$lAgrosphäre$$x0
000017113 970__ $$aVDB:(DE-Juel1)131507
000017113 980__ $$aVDB
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000017113 980__ $$aI:(DE-Juel1)IBG-3-20101118
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