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@INPROCEEDINGS{Metzner:171783,
author = {Metzner, Ralf},
title = {{M}onitoring roots, nodule and pod development in vivo:
{N}ew perspectives on legume development},
reportid = {FZJ-2014-05347},
year = {2014},
abstract = {Seed filling, root and nodule development are some of the
key processes involved in abiotic stress resistance.
However, these are complex processes hidden either by pod
tissue (seed filling) or by soil (roots and nodules), so
studying them quantitatively in-vivo is challenging.
Non-invasive (3D) imaging techniques such as magnetic
resonance imaging (MRI) can be used to investigate hidden
structural development both above- and belowground.
Co-registered positron emission tomography (PET) allows the
acquisition of functional information by mapping of recent
carbon investment of the plant and its dynamics e.g. in
response to stress. For tasks not requiring spatial
(volumetric) resolution, such as monitoring changes in pod
water and dry-matter content, low field nuclear magnetic
resonance relaxometry with portable devices (pNMR) can be
applied. Such devices allow sensor-like applications in the
greenhouse and the field. In the current contribution we
will demonstrate the application of MRI to follow the
development of root system architecture in soil grown pea
and bean genotypes and monitor the progress of nodulation by
repeated 3D mapping of nodule distribution. By correlating
these maps with PET, monitoring carbon import, we get first
insights into the functionality of nodules in the soil.
Additionally we will demonstrate monitoring changes of bean
pod water and dry-matter contents over the course of several
weeks with pNMR. We discuss the potential and challenges of
all three techniques (MRI, PET and pNMR) for application in
legume research.},
month = {Jul},
date = {2014-07-06},
organization = {The 6th International Food Legumes
Research Conference, Saskatoon
(Canada), 6 Jul 2014 - 11 Jul 2014},
subtyp = {Other},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {242 - Sustainable Bioproduction (POF2-242) / BMBF-0315532A
- CROP.SENSe.net (BMBF-0315532A) / DPPN - Deutsches Pflanzen
Phänotypisierungsnetzwerk (BMBF-031A053A)},
pid = {G:(DE-HGF)POF2-242 / G:(DE-Juel1)BMBF-0315532A /
G:(DE-Juel1)BMBF-031A053A},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/171783},
}