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@ARTICLE{McDaniel:171844,
author = {McDaniel, Jonathan R. and Weitzhandler, Isaac and Prevost,
Sylvain and Vargo, Kevin B. and Appavou, Marie-Sousai and
Hammer, Daniel A. and Gradzielski, Michael and Chilkoti,
Ashutosh},
title = {{N}oncanonical {S}elf-{A}ssembly of {H}ighly {A}symmetric
{G}enetically {E}ncoded {P}olypeptide {A}mphiphiles into
{C}ylindrical {M}icelles},
journal = {Nano letters},
volume = {14},
number = {11},
issn = {1530-6992},
address = {Washington, DC},
publisher = {ACS Publ.},
reportid = {FZJ-2014-05401},
pages = {6590–6598},
year = {2014},
abstract = {Elastin-like polypeptides (ELPs) are a class of biopolymers
consisting of the pentameric repeat (VPGαG)n based on the
sequence of mammalian tropoelastin that display a thermally
induced soluble-to-insoluble phase transition in aqueous
solution. We have discovered a remarkably simple approach to
driving the spontaneous self-assembly of high molecular
weight ELPs into nanostructures by genetically fusing a
short 1.5 kDa (XGy)z assembly domain to one end of the ELP.
Classical theories of self-assembly based on the geometric
mass balance of hydrophilic and hydrophobic block copolymers
suggest that these highly asymmetric polypeptides should
form spherical micelles. Surprisingly, when sufficiently
hydrophobic amino acids (X) are presented in a periodic
sequence such as (FGG)8 or (YG)8, these highly asymmetric
polypeptides self-assemble into cylindrical micelles whose
length can be tuned by the sequence of the morphogenic tag.
These nanostructures were characterized by light scattering,
tunable resistive pulse sensing, fluorescence
spectrophotometry, and thermal turbidimetry, as well as by
cryogenic transmission electron microscopy (cryo-TEM) and
small-angle neutron scattering (SANS). These short assembly
domains provide a facile strategy to control the size,
shape, and stability of stimuli responsive polypeptide
nanostructures.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218},
pnm = {54G - JCNS (POF2-54G24)},
pid = {G:(DE-HGF)POF2-54G24},
experiment = {EXP:(DE-MLZ)KWS3-20140101 / EXP:(DE-MLZ)KWS1-20140101 /
EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000345723800089},
pubmed = {pmid:25268037},
doi = {10.1021/nl503221p},
url = {https://juser.fz-juelich.de/record/171844},
}