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000172157 037__ $$aFZJ-2014-05667
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000172157 1001_ $$0P:(DE-HGF)0$$aMirecka, Ewa Agnieszka$$b0
000172157 245__ $$aEngineered aggregation inhibitor fusion for production of highly amyloidogenic human islet amyloid polypeptide
000172157 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2014
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000172157 520__ $$aHuman islet amyloid polypeptide (IAPP) is the major component of pancreatic amyloid deposits in type 2 diabetes. The structural conversion of IAPP from a monomeric state into amyloid assemblies is the subject of intense research. Recombinant production of IAPP is, however, difficult due to its extreme aggregation propensity. Here we describe a novel strategy for expression of IAPP in Escherichia coli, based on an engineered protein tag, which sequesters IAPP monomers and prevents IAPP aggregation. The IAPP-binding protein HI18 was selected by phage display from a β-wrapin library. Fusion of HI18 to IAPP enabled the soluble expression of the construct. IAPP was cleaved from the fusion construct and purified to homogeneity with a yield of 3mg of isotopically labeled peptide per liter of culture. In the monomeric state, IAPP was largely disordered as evidenced by far-UV CD and liquid-state NMR spectroscopy but competent to form amyloid fibrils according to atomic force microscopy. These results demonstrate the ability of the engineered β-wrapin HI18 for shielding the hydrophobic sequence of IAPP during expression and purification. Fusion of aggregation-inhibiting β-wrapins is a suitable approach for the recombinant production of aggregation-prone proteins.
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000172157 7001_ $$0P:(DE-Juel1)145165$$aGremer, Lothar$$b1$$ufzj
000172157 7001_ $$0P:(DE-HGF)0$$aSchiefer, Stephanie$$b2
000172157 7001_ $$0P:(DE-HGF)0$$aOesterhelt, Filipp$$b3
000172157 7001_ $$0P:(DE-Juel1)132023$$aStoldt, Matthias$$b4$$ufzj
000172157 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b5$$ufzj
000172157 7001_ $$0P:(DE-HGF)0$$aHoyer, Wolfgang$$b6$$eCorresponding Author
000172157 773__ $$0PERI:(DE-600)2016476-2$$a10.1016/j.jbiotec.2014.06.006$$gp. S0168165614002879$$pS0168-1656(14)00287-9$$tJournal of biotechnology$$v-$$x0168-1656$$y2014
000172157 8564_ $$uhttp://www.sciencedirect.com/science/article/pii/S0168165614002879
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