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@ARTICLE{Keuters:173157,
author = {Keuters, M. H. and Aswendt, M. and Tennstaedt, A. and
Wiedermann, D. and Pikhovych, S. and Rotthues, S. and Fink,
Gereon Rudolf and Schroeter, M. and Hoehn, M. and Rueger, M.
A.},
title = {{T}ranscranial direct current stimulation promotes the
mobility of engrafted neural stem cells in the rat brain},
journal = {NMR in biomedicine},
volume = {28},
number = {2},
issn = {0952-3480},
address = {New York, NY},
publisher = {Wiley},
reportid = {FZJ-2014-06570},
pages = {231 - 239},
year = {2015},
abstract = {Transcranial direct current stimulation (tDCS) is used in
numerous clinical studies and considered an effective and
versatile add-on therapy in neurorehabilitation. To date,
however, the underlying neurobiological mechanisms remain
elusive. In a rat model of tDCS, we recently observed a
polarity-dependent accumulation of endogenous neural stem
cells (NSCs) in the stimulated cortex. Based upon these
findings, we hypothesized that tDCS may exert a direct
migratory effect on endogenous NSCs towards the stimulated
cortex. Using noninvasive imaging, we here investigated
whether tDCS may also cause a directed migration of
engrafted NSCs. Murine NSCs were labeled with
superparamagnetic particles of iron oxide (SPIOs) and
implanted into rat striatum and corpus callosum. MRI was
performed (i) immediately after implantation and (ii) after
10 tDCS sessions of anodal or cathodal polarity.
Sham-stimulated rats served as control. Imaging results were
validated ex vivo using immunohistochemistry. Overall
migratory activity of NSCs almost doubled after anodal tDCS.
However, no directed migration within the electric field
(i.e. towards or away from the electrode) could be observed.
Rather, an undirected outward migration from the center of
the graft was detected. Xenograft transplantation induced a
neuroinflammatory response that was significantly enhanced
following cathodal tDCS. This inflammatory response did not
impact negatively on the survival of implanted NSCs. Data
suggest that anodal tDCS increases the undirected migratory
activity of implanted NSCs. Since the electric field did not
guide implanted NSCs over large distances, previously
observed polarity-dependent accumulation of endogenous NSCs
in the cortex might have originated from local
proliferation. Results enhance our understanding of the
neurobiological mechanisms underlying tDCS, and may thereby
help to develop a targeted and sustainable application of
tDCS in clinical practice},
cin = {INM-3},
ddc = {610},
cid = {I:(DE-Juel1)INM-3-20090406},
pnm = {572 - (Dys-)function and Plasticity (POF3-572)},
pid = {G:(DE-HGF)POF3-572},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000349070900010},
pubmed = {pmid:25521600},
doi = {10.1002/nbm.3244},
url = {https://juser.fz-juelich.de/record/173157},
}