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000173322 1001_ $$0P:(DE-Juel1)132017$$aMüller, Henrik$$b0
000173322 245__ $$aProgress towards structural understanding of infectious sheep PrP-amyloid
000173322 260__ $$aAustin, Tex.$$bLandes Bioscience$$c2014
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000173322 520__ $$aThe still elusive structural difference of non-infectious and infectious amyloid of the mammalian prion protein (PrP) is a major pending milestone in understanding protein-mediated infectivity in neurodegenerative diseases. Preparations of PrP-amyloid proven to be infectious have never been investigated with a high-resolution technique. All available models to date have been based on low-resolution data. Here, we establish protocols for the preparation of infectious samples of full-length recombinant (rec) PrP¡amyloid in NMR-sufficient amounts by spontaneous fibrillation and seeded fibril growth from brain extract. We link biological and structural data of infectious recPrP-amyloid, derived from bioassays, atomic force microscopy, and solid-state NMR spectroscopy. Our data indicate a semi-mobile N‑terminus, some residues with secondary chemical shifts typical of α‑helical secondary structure in the middle part between ~115 to ~155, and a distinct β‑sheet core C‑terminal of residue ~155. These findings are not in agreement with all current models for PrP-amyloid. We also provide evidence that samples seeded from brain extract may not differ in the overall arrangement of secondary structure elements, but rather in the flexibility of protein segments outside the β-core region. Taken together, our protocols provide an essential basis for the high-resolution characterization of non-infectious and infectious PrP-amyloid in the near future.
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000173322 7001_ $$0P:(DE-HGF)0$$aBrener, Oleksandr$$b1
000173322 7001_ $$0P:(DE-HGF)0$$aAndreoletti, Olivier$$b2
000173322 7001_ $$0P:(DE-Juel1)145776$$aPiechatzek, Timo$$b3$$ufzj
000173322 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b4$$ufzj
000173322 7001_ $$0P:(DE-HGF)0$$aLegname, Giuseppe$$b5
000173322 7001_ $$0P:(DE-Juel1)132002$$aHeise, Henrike$$b6$$eCorresponding Author$$ufzj
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