%0 Journal Article
%A Beisel, K.G.
%A Schurr, U.
%A Matsubara, S.
%T Altered Turnover of beta-Carotene and Chl a in Arabidopsis Leaves Treated with Lincomycin or Norflurazon
%J Plant & cell physiology
%V 52
%@ 0032-0781
%C Oxford
%I Oxford University Press
%M PreJuSER-17572
%P 1193 - 1203
%D 2011
%Z Record converted from VDB: 12.11.2012
%X Interactions between β-carotene (β-C) and Chl a turnover were investigated in relation to photoinhibition and D1 protein turnover in mature leaves of Arabidopsis (Arabidopsis thaliana) by ¹⁴CO₂ pulse-chase labeling. Following a 2 h treatment of leaves with water, lincomycin (Linco; an inhibitor of chloroplast protein synthesis) or norflurazon (NF; an inhibitor of carotenoid biosynthesis at phytoene desaturation) in the dark, ¹⁴CO₂ was applied to the leaves for 30 min under control light (CL; 130 μmol photons m⁻² s⁻¹) conditions, followed by exposure to either CL or high light (HL; 1,100 μmol photons m⁻² s⁻¹) in ambient CO₂ for up to 6 h. Under both light conditions, ¹⁴C incorporation was strongly decreased for Chl a and moderately suppressed for β-C in Linco-treated leaves, showing a marked decline of PSII efficiency (F(v)/F(m)) and β-C content compared with water-treated leaves. Partial inhibition of carotenoid biosynthesis by NF caused no or only a minor decrease in F(v)/F(m) and Chl a turnover under both conditions, while the β-C content significantly declined and high ¹⁴C labeling was found for phytoene, the substrate of phytoene desaturase. Together, the results suggest coordinated turnover of Chl a and D1, but somewhat different regulation for β-C turnover, in Arabidopsis leaves. Inhibition of carotenoid biosynthesis by NF may initially enhance metabolic flux in the pathway upstream of phytoene, presumably compensating for short supply of β-C. Our observations are also in line with the notion that HL-induced accumulation of xanthophylls may involve a precursor pool which is distinct from that for β-C turnover.
%K Arabidopsis: metabolism
%K Carbon Dioxide: metabolism
%K Carotenoids: metabolism
%K Chlorophyll: biosynthesis
%K Isotope Labeling
%K Light
%K Lincomycin: pharmacology
%K Photosystem II Protein Complex: metabolism
%K Plant Leaves: metabolism
%K Pyridazines: pharmacology
%K beta Carotene: biosynthesis
%K Photosystem II Protein Complex (NLM Chemicals)
%K Pyridazines (NLM Chemicals)
%K Carbon Dioxide (NLM Chemicals)
%K Chlorophyll (NLM Chemicals)
%K Lincomycin (NLM Chemicals)
%K norflurazone (NLM Chemicals)
%K Carotenoids (NLM Chemicals)
%K chlorophyll a (NLM Chemicals)
%K phytoene (NLM Chemicals)
%K beta Carotene (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:21632655
%U <Go to ISI:>//WOS:000292837500006
%R 10.1093/pcp/pcr069
%U https://juser.fz-juelich.de/record/17572