TY  - JOUR
AU  - Beisel, K.G.
AU  - Schurr, U.
AU  - Matsubara, S.
TI  - Altered Turnover of beta-Carotene and Chl a in Arabidopsis Leaves Treated with Lincomycin or Norflurazon
JO  - Plant & cell physiology
VL  - 52
SN  - 0032-0781
CY  - Oxford
PB  - Oxford University Press
M1  - PreJuSER-17572
SP  - 1193 - 1203
PY  - 2011
N1  - Record converted from VDB: 12.11.2012
AB  - Interactions between β-carotene (β-C) and Chl a turnover were investigated in relation to photoinhibition and D1 protein turnover in mature leaves of Arabidopsis (Arabidopsis thaliana) by ¹⁴CO₂ pulse-chase labeling. Following a 2 h treatment of leaves with water, lincomycin (Linco; an inhibitor of chloroplast protein synthesis) or norflurazon (NF; an inhibitor of carotenoid biosynthesis at phytoene desaturation) in the dark, ¹⁴CO₂ was applied to the leaves for 30 min under control light (CL; 130 μmol photons m⁻² s⁻¹) conditions, followed by exposure to either CL or high light (HL; 1,100 μmol photons m⁻² s⁻¹) in ambient CO₂ for up to 6 h. Under both light conditions, ¹⁴C incorporation was strongly decreased for Chl a and moderately suppressed for β-C in Linco-treated leaves, showing a marked decline of PSII efficiency (F(v)/F(m)) and β-C content compared with water-treated leaves. Partial inhibition of carotenoid biosynthesis by NF caused no or only a minor decrease in F(v)/F(m) and Chl a turnover under both conditions, while the β-C content significantly declined and high ¹⁴C labeling was found for phytoene, the substrate of phytoene desaturase. Together, the results suggest coordinated turnover of Chl a and D1, but somewhat different regulation for β-C turnover, in Arabidopsis leaves. Inhibition of carotenoid biosynthesis by NF may initially enhance metabolic flux in the pathway upstream of phytoene, presumably compensating for short supply of β-C. Our observations are also in line with the notion that HL-induced accumulation of xanthophylls may involve a precursor pool which is distinct from that for β-C turnover.
KW  - Arabidopsis: metabolism
KW  - Carbon Dioxide: metabolism
KW  - Carotenoids: metabolism
KW  - Chlorophyll: biosynthesis
KW  - Isotope Labeling
KW  - Light
KW  - Lincomycin: pharmacology
KW  - Photosystem II Protein Complex: metabolism
KW  - Plant Leaves: metabolism
KW  - Pyridazines: pharmacology
KW  - beta Carotene: biosynthesis
KW  - Photosystem II Protein Complex (NLM Chemicals)
KW  - Pyridazines (NLM Chemicals)
KW  - Carbon Dioxide (NLM Chemicals)
KW  - Chlorophyll (NLM Chemicals)
KW  - Lincomycin (NLM Chemicals)
KW  - norflurazone (NLM Chemicals)
KW  - Carotenoids (NLM Chemicals)
KW  - chlorophyll a (NLM Chemicals)
KW  - phytoene (NLM Chemicals)
KW  - beta Carotene (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21632655
UR  - <Go to ISI:>//WOS:000292837500006
DO  - DOI:10.1093/pcp/pcr069
UR  - https://juser.fz-juelich.de/record/17572
ER  -