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@ARTICLE{Beisel:17572,
author = {Beisel, K.G. and Schurr, U. and Matsubara, S.},
title = {{A}ltered {T}urnover of beta-{C}arotene and {C}hl a in
{A}rabidopsis {L}eaves {T}reated with {L}incomycin or
{N}orflurazon},
journal = {Plant $\&$ cell physiology},
volume = {52},
issn = {0032-0781},
address = {Oxford},
publisher = {Oxford University Press},
reportid = {PreJuSER-17572},
pages = {1193 - 1203},
year = {2011},
note = {Record converted from VDB: 12.11.2012},
abstract = {Interactions between β-carotene (β-C) and Chl a turnover
were investigated in relation to photoinhibition and D1
protein turnover in mature leaves of Arabidopsis
(Arabidopsis thaliana) by ¹⁴CO₂ pulse-chase labeling.
Following a 2 h treatment of leaves with water, lincomycin
(Linco; an inhibitor of chloroplast protein synthesis) or
norflurazon (NF; an inhibitor of carotenoid biosynthesis at
phytoene desaturation) in the dark, ¹⁴CO₂ was applied
to the leaves for 30 min under control light (CL; 130 μmol
photons m⁻² s⁻¹) conditions, followed by exposure to
either CL or high light (HL; 1,100 μmol photons m⁻²
s⁻¹) in ambient CO₂ for up to 6 h. Under both light
conditions, ¹⁴C incorporation was strongly decreased for
Chl a and moderately suppressed for β-C in Linco-treated
leaves, showing a marked decline of PSII efficiency
(F(v)/F(m)) and β-C content compared with water-treated
leaves. Partial inhibition of carotenoid biosynthesis by NF
caused no or only a minor decrease in F(v)/F(m) and Chl a
turnover under both conditions, while the β-C content
significantly declined and high ¹⁴C labeling was found
for phytoene, the substrate of phytoene desaturase.
Together, the results suggest coordinated turnover of Chl a
and D1, but somewhat different regulation for β-C turnover,
in Arabidopsis leaves. Inhibition of carotenoid biosynthesis
by NF may initially enhance metabolic flux in the pathway
upstream of phytoene, presumably compensating for short
supply of β-C. Our observations are also in line with the
notion that HL-induced accumulation of xanthophylls may
involve a precursor pool which is distinct from that for
β-C turnover.},
keywords = {Arabidopsis: metabolism / Carbon Dioxide: metabolism /
Carotenoids: metabolism / Chlorophyll: biosynthesis /
Isotope Labeling / Light / Lincomycin: pharmacology /
Photosystem II Protein Complex: metabolism / Plant Leaves:
metabolism / Pyridazines: pharmacology / beta Carotene:
biosynthesis / Photosystem II Protein Complex (NLM
Chemicals) / Pyridazines (NLM Chemicals) / Carbon Dioxide
(NLM Chemicals) / Chlorophyll (NLM Chemicals) / Lincomycin
(NLM Chemicals) / norflurazone (NLM Chemicals) / Carotenoids
(NLM Chemicals) / chlorophyll a (NLM Chemicals) / phytoene
(NLM Chemicals) / beta Carotene (NLM Chemicals) / J
(WoSType)},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences / Cell Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21632655},
UT = {WOS:000292837500006},
doi = {10.1093/pcp/pcr069},
url = {https://juser.fz-juelich.de/record/17572},
}