TY  - JOUR
AU  - Kisner, A.
AU  - Stockmann, R.
AU  - Jansen, M.
AU  - Yegin, U.
AU  - Offenhäusser, A.
AU  - Kubota, L. T.
AU  - Mourzina, Y.
TI  - Sensing small neurotransmitter enzyme interaction with nanoporous gated ion-sensitive field effect transistors
JO  - Biosensors and bioelectronics
VL  - 31
IS  - 1
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-17994
SP  - 157–163
PY  - 2012
N1  - Record converted from VDB: 12.11.2012
AB  - Ion-sensitive field effect transistors with gates having a high density of nanopores were fabricated and employed to sense the neurotransmitter dopamine with high selectivity and detectability at micromolar range. The nanoporous structure of the gates was produced by applying a relatively simple anodizing process, which yielded a porous alumina layer with pores exhibiting a mean diameter ranging from 20 to 35 nm. Gate-source voltages of the transistors demonstrated a pH-dependence that was linear over a wide range and could be understood as changes in surface charges during protonation and deprotonation. The large surface area provided by the pores allowed the physical immobilization of tyrosinase, which is an enzyme that oxidizes dopamine, on the gates of the transistors, and thus, changes the acid-base behavior on their surfaces. Concentration-dependent dopamine interacting with immobilized tyrosinase showed a linear dependence into a physiological range of interest for dopamine concentration in the changes of gate-source voltages. In comparison with previous approaches, a response time relatively fast for detecting dopamine was obtained. Additionally, selectivity assays for other neurotransmitters that are abundantly found in the brain were examined. These results demonstrate that the nanoporous structure of ion-sensitive field effect transistors can easily be used to immobilize specific enzyme that can readily and selectively detect small neurotransmitter molecule based on its acid-base interaction with the receptor. Therefore, it could serve as a technology platform for molecular studies of neurotransmitter-enzyme binding and drugs screening.
KW  - Biosensing Techniques: instrumentation
KW  - Conductometry: instrumentation
KW  - Electrodes
KW  - Enzymes, Immobilized: chemistry
KW  - Equipment Design
KW  - Equipment Failure Analysis
KW  - Ions
KW  - Monophenol Monooxygenase: chemistry
KW  - Nanostructures: chemistry
KW  - Nanostructures: ultrastructure
KW  - Nanotechnology: instrumentation
KW  - Neurotransmitter Agents: chemistry
KW  - Porosity
KW  - Protein Binding
KW  - Protein Interaction Mapping: instrumentation
KW  - Reproducibility of Results
KW  - Sensitivity and Specificity
KW  - Transistors, Electronic
KW  - Enzymes, Immobilized (NLM Chemicals)
KW  - Ions (NLM Chemicals)
KW  - Neurotransmitter Agents (NLM Chemicals)
KW  - Monophenol Monooxygenase (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22040747
UR  - <Go to ISI:>//WOS:000300468400025
DO  - DOI:10.1016/j.bios.2011.10.010
UR  - https://juser.fz-juelich.de/record/17994
ER  -