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@ARTICLE{Kisner:17994,
      author       = {Kisner, A. and Stockmann, R. and Jansen, M. and Yegin, U.
                      and Offenhäusser, A. and Kubota, L. T. and Mourzina, Y.},
      title        = {{S}ensing small neurotransmitter enzyme interaction with
                      nanoporous gated ion-sensitive field effect transistors},
      journal      = {Biosensors and bioelectronics},
      volume       = {31},
      number       = {1},
      issn         = {0956-5663},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {PreJuSER-17994},
      pages        = {157–163},
      year         = {2012},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Ion-sensitive field effect transistors with gates having a
                      high density of nanopores were fabricated and employed to
                      sense the neurotransmitter dopamine with high selectivity
                      and detectability at micromolar range. The nanoporous
                      structure of the gates was produced by applying a relatively
                      simple anodizing process, which yielded a porous alumina
                      layer with pores exhibiting a mean diameter ranging from 20
                      to 35 nm. Gate-source voltages of the transistors
                      demonstrated a pH-dependence that was linear over a wide
                      range and could be understood as changes in surface charges
                      during protonation and deprotonation. The large surface area
                      provided by the pores allowed the physical immobilization of
                      tyrosinase, which is an enzyme that oxidizes dopamine, on
                      the gates of the transistors, and thus, changes the
                      acid-base behavior on their surfaces.
                      Concentration-dependent dopamine interacting with
                      immobilized tyrosinase showed a linear dependence into a
                      physiological range of interest for dopamine concentration
                      in the changes of gate-source voltages. In comparison with
                      previous approaches, a response time relatively fast for
                      detecting dopamine was obtained. Additionally, selectivity
                      assays for other neurotransmitters that are abundantly found
                      in the brain were examined. These results demonstrate that
                      the nanoporous structure of ion-sensitive field effect
                      transistors can easily be used to immobilize specific enzyme
                      that can readily and selectively detect small
                      neurotransmitter molecule based on its acid-base interaction
                      with the receptor. Therefore, it could serve as a technology
                      platform for molecular studies of neurotransmitter-enzyme
                      binding and drugs screening.},
      keywords     = {Biosensing Techniques: instrumentation / Conductometry:
                      instrumentation / Electrodes / Enzymes, Immobilized:
                      chemistry / Equipment Design / Equipment Failure Analysis /
                      Ions / Monophenol Monooxygenase: chemistry / Nanostructures:
                      chemistry / Nanostructures: ultrastructure / Nanotechnology:
                      instrumentation / Neurotransmitter Agents: chemistry /
                      Porosity / Protein Binding / Protein Interaction Mapping:
                      instrumentation / Reproducibility of Results / Sensitivity
                      and Specificity / Transistors, Electronic / Enzymes,
                      Immobilized (NLM Chemicals) / Ions (NLM Chemicals) /
                      Neurotransmitter Agents (NLM Chemicals) / Monophenol
                      Monooxygenase (NLM Chemicals)},
      cin          = {ICS-8 / JARA-FIT / PGI-8},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / $I:(DE-82)080009_20140620$ /
                      I:(DE-Juel1)PGI-8-20110106},
      pnm          = {Grundlagen für zukünftige Informationstechnologien /
                      BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung},
      pid          = {G:(DE-Juel1)FUEK412 / G:(DE-Juel1)FUEK505},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22040747},
      UT           = {WOS:000300468400025},
      doi          = {10.1016/j.bios.2011.10.010},
      url          = {https://juser.fz-juelich.de/record/17994},
}