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000018193 0247_ $$2DOI$$a10.1021/bm201184w
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000018193 084__ $$2WoS$$aBiochemistry & Molecular Biology
000018193 084__ $$2WoS$$aChemistry, Organic
000018193 084__ $$2WoS$$aPolymer Science
000018193 1001_ $$0P:(DE-HGF)0$$aPrevost, S.$$b0
000018193 245__ $$aColloidal Structure and Stability of DNA/Polycations Polyplexes investigated by Small Angle Scattering
000018193 260__ $$aColumbus, Ohio$$bAmerican Chemical Soc.$$c2011
000018193 300__ $$a4272
000018193 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000018193 440_0 $$08221$$aBiomacromolecules$$v12$$x1525-7797$$y12
000018193 500__ $$aFor allocation of SANS and SAXS beam times, we are grateful to the HZB, ILL, JCNS, and ESRF. For help with the scattering experiments, we would like to thank T. Narayanan (ESRF, ID2). For her constant help during the SANS and SAXS measurements, we are indebted to P. Heunemann. N. Graeber and A. Walther are thanked for preparation of samples. SANS experiments at the HZB and JCNS have been supported by the European Commission under the 7th Framework Program through the Key Action: Strengthening the European Research Area, Research Infrastructures, Contract No. 226 507 (NMI3). The research project has been supported and funded by the SFB 448 of the Deutsche Forschungsgemeinschaft.
000018193 520__ $$aPolyplexes of short DNA-fragments (300 b.p., 100 nm) with tailor-made amine-based polycations of different architectures (linear and hyperbranched) were investigated in buffer solution as a function of the mixing ratio with DNA. The resulting dispersed polyplexes were characterized using small-angle neutron and X-ray scattering (SANS, SAXS) as well as cryo-TEM with respect to their mesoscopic structure and their colloidal stability. The linear polyimines form rather compact structures that have a high tendency for precipitation. In contrast, the hyperbranched polycation with enzymatic-labile pentaethylenehexamine arms (PEHA) yields polyplexes colloidally stable for months. Here the polycation coating of DNA results in a homogeneous dispersion based on a fractal network with low structural organization at low polycation amount. With increasing polycation, bundles of tens of aligned DNA rods appear that are interconnected in a fractal network with a typical correlation distance on the order of 100 nm, the average length of the DNA used. With higher organization comes a decrease in stability. The 3D network built by these beams can still exhibit some stability as long as the material concentration is large enough, but the structure collapses upon dilution. SAXS shows that the complexation does not affect the local DNA structure. Interestingly, the structural findings on the DNA polyplexes apparently correlate with the transfection efficiency of corresponding siRNA complexes. In general, these finding not only show systematic trends for the colloid stability, but may allow for rational approaches to design effective transfection carriers.
000018193 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000018193 536__ $$0G:(DE-Juel1)FUEK415$$2G:(DE-HGF)$$aGroßgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI)$$cP55$$x1
000018193 588__ $$aDataset connected to Web of Science, Pubmed
000018193 650_2 $$2MeSH$$aColloids: chemistry
000018193 650_2 $$2MeSH$$aDNA: chemistry
000018193 650_2 $$2MeSH$$aDNA: metabolism
000018193 650_2 $$2MeSH$$aDNA: ultrastructure
000018193 650_2 $$2MeSH$$aPolyamines: chemistry
000018193 650_2 $$2MeSH$$aPolymers: chemistry
000018193 650_2 $$2MeSH$$aScattering, Small Angle
000018193 650_7 $$00$$2NLM Chemicals$$aColloids
000018193 650_7 $$00$$2NLM Chemicals$$aPolyamines
000018193 650_7 $$00$$2NLM Chemicals$$aPolymers
000018193 650_7 $$00$$2NLM Chemicals$$apolycations
000018193 650_7 $$04067-16-7$$2NLM Chemicals$$apentaethylenehexamine
000018193 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
000018193 650_7 $$2WoSType$$aJ
000018193 65027 $$0V:(DE-MLZ)SciArea-210$$2V:(DE-HGF)$$aSoft Condensed Matter$$x0
000018193 65017 $$0V:(DE-MLZ)GC-130-2016$$2V:(DE-HGF)$$aHealth and Life$$x1
000018193 65017 $$0V:(DE-MLZ)GC-130-1$$2V:(DE-HGF)$$aHealth and Life$$x0
000018193 693__ $$0EXP:(DE-MLZ)KWS2-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)KWS2-20140101$$6EXP:(DE-MLZ)NL3ao-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz$$eKWS-2: Small angle scattering diffractometer$$fNL3ao$$x0
000018193 7001_ $$0P:(DE-HGF)0$$aRiemer, S.$$b1
000018193 7001_ $$0P:(DE-HGF)0$$aFischer, W.$$b2
000018193 7001_ $$0P:(DE-HGF)0$$aHaag, R.$$b3
000018193 7001_ $$0P:(DE-HGF)0$$aBöttcher, C.$$b4
000018193 7001_ $$0P:(DE-HGF)0$$aGummel, J.$$b5
000018193 7001_ $$0P:(DE-HGF)0$$aGrillo, I.$$b6
000018193 7001_ $$0P:(DE-Juel1)VDB95094$$aAppavou, M.-S.$$b7$$uFZJ
000018193 7001_ $$0P:(DE-HGF)0$$aGradzielski, M.$$b8
000018193 773__ $$0PERI:(DE-600)2006291-6$$a10.1021/bm201184w$$gVol. 12, p. 4272$$p4272$$q12<4272$$tBiomacromolecules$$v12$$x1525-7797$$y2011
000018193 8567_ $$uhttp://dx.doi.org/10.1021/bm201184w
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