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@ARTICLE{Appavou:18195,
      author       = {Appavou, M-S. and Busch, S. and Doster, W. and Gaspar, A.
                      and Unruh, T.},
      title        = {{T}he influence of 2 kbar pressure on the global and
                      internal dynamics of human hemoglobin observed by
                      quasielastic neutron scattering},
      journal      = {European biophysics journal},
      volume       = {40},
      issn         = {0175-7571},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-18195},
      pages        = {705 - 714},
      year         = {2011},
      note         = {The research project was supported by a grant of the
                      Deutsche Forschungsgemeinschaft, SFB 533, TP B11, which is
                      gratefully acknowledged. We would like to acknowledge
                      particularly Joachim Dorbecker and Reinhold Funer who helped
                      to build the 2 kbar pressure cell and the platform for
                      TOFTOF. We would like to thank Jandal Ringe for his help on
                      TOFTOF during our experiments. The ANTARES team, especially
                      Martin Muhlbauer and Elbio Calzada, are thanked for allowing
                      us to use some beam time for neutronography measurements,
                      and to Robert Georgii for beamtime on MIRA to perform the
                      SANS characterization measurements. Finally, we would like
                      to thank Alan Soper for useful discussions.},
      comment      = {..},
      booktitle     = {..},
      abstract     = {Pressure is a ubiquitous physical parameter in life and is
                      commonly used in the life sciences to study new protein
                      folding pathways or association-dissociation phenomena. In
                      this paper, an investigation of the influence of pressure on
                      hemoglobin, a multimeric protein, at the picosecond time
                      scale is presented using time-of-flight neutron scattering.
                      The aim is to observe the influence of pressure on the
                      translational diffusion and internal motions of hemoglobin
                      in a concentrated solution and a possible dissociation of
                      the subunits as suggested by Pin et al. (Biochemistry
                      29:9194, 1990) using fluorescence spectroscopy. A new flat 2
                      kbar pressure cell made of an aluminum alloy has been used,
                      which allowed the effect of pressure to be studied with
                      minimum background contribution. Within this range of
                      pressure, the effect of this physical parameter on global
                      diffusion can be explained in terms of the change in the
                      water buffer viscosity and an oligomerization of hemoglobin
                      subunits, whereas the internal motions were less affected.},
      keywords     = {Diffusion / Hemoglobin Subunits: chemistry / Humans /
                      Motion / Neutron Diffraction: methods / Pressure / Protein
                      Conformation / Scattering, Radiation / Viscosity /
                      Hemoglobin Subunits (NLM Chemicals) / J (WoSType)},
      cin          = {ICS-1 / JCNS (München) ; Jülich Centre for Neutron
                      Science JCNS (München) ; JCNS-FRM-II / JCNS-1 / JCNS-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-1-20110106 /
                      I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-2-20110106},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung / Großgeräte für die Forschung
                      mit Photonen, Neutronen und Ionen (PNI)},
      pid          = {G:(DE-Juel1)FUEK505 / G:(DE-Juel1)FUEK415},
      experiment   = {EXP:(DE-MLZ)TOF-TOF-20140101},
      shelfmark    = {Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21340585},
      UT           = {WOS:000290331400001},
      doi          = {10.1007/s00249-011-0678-3},
      url          = {https://juser.fz-juelich.de/record/18195},
}