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000018198 0247_ $$2pmid$$apmid:21736300
000018198 0247_ $$2pmc$$apmc:PMC3170880
000018198 0247_ $$2DOI$$a10.1021/ja202622g
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000018198 084__ $$2WoS$$aChemistry, Multidisciplinary
000018198 1001_ $$0P:(DE-HGF)0$$aWolf, S.$$b0
000018198 245__ $$aStrong stabilization of liquid amorphous calcium carbonate by ovalbumin: gaining insight into the mechanism of 'polymer-induced liquid precursor' processes
000018198 260__ $$aWashington, DC$$bAmerican Chemical Society$$c2011
000018198 300__ $$a12642 - 12649
000018198 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000018198 440_0 $$021462$$aJournal of the American Chemical Society$$v133$$x0002-7863$$y32
000018198 500__ $$aWe thank Simone Rolf and Ralf Bienert (BAM) for technical support at the beamline. We are much obliged to Frederic Mann and Ute Schlossmacher for assistance in IG labeling and fruitful discussions. We are grateful to the Deutsche Forschungsgemeinschaft (DFG) for support within the priority program No. 1415: "Kristalline Nichtgleichgewichtsphasen". S.E.W.gratefully acknowledges the Konrad Adenauer Foundation for a doctoral fellowship. We thank the BioCAT team at the Advanced Photon Source for excellent support during XAS experiments, and we are grateful to Dr. David Gore for his careful revision of the English style. Use of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE), Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357. BioCAT is a National Institutes of Health-supported Research Center RR-08630.
000018198 520__ $$aThe impact of the ovo proteins ovalbumin and lysozyme--present in the first stage of egg shell formation--on the homogeneous formation of the liquid amorphous calcium carbonate (LACC) precursor, was studied by a combination of complementing methods: in situ WAXS, SANS, XANES, TEM, and immunogold labeling. Lysozyme (pI = 9.3) destabilizes the LACC emulsion whereas the glycoprotein ovalbumin (pI = 4.7) extends the lifespan of the emulsified state remarkably. In the light of the presented data: (a) Ovalbumin is shown to behave commensurable to the 'polymer-induced liquid precursor' (PILP) process proposed by Gower et al. Ovalbumin can be assumed to take a key role during eggshell formation where it serves as an effective stabilization agent for transient precursors and prevents undirected mineralization of the eggshell. (b) It is further shown that the emulsified LACC carries a negative surface charge and is electrostatically stabilized. (c) We propose that the liquid amorphous calcium carbonate is affected by polymers by depletion stabilization and de-emulsification rather than 'induced' by acidic proteins and polymers during a so-called polymer-induced liquid-precursor process. The original PILP coating effect, first reported by Gower et al., appears to be a result of a de-emulsification process of a stabilized LACC phase. The behavior of the liquid amorphous carbonate phase and the polymer-induced liquid-precursor phase itself can be well described by colloid chemical terms: electrostatic and depletion stabilization and de-emulsification by depletion destabilization.
000018198 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000018198 536__ $$0G:(DE-Juel1)FUEK415$$2G:(DE-HGF)$$aGroßgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI)$$cP55$$x1
000018198 588__ $$aDataset connected to Web of Science, Pubmed
000018198 650_2 $$2MeSH$$aAnimals
000018198 650_2 $$2MeSH$$aCalcium Carbonate: chemistry
000018198 650_2 $$2MeSH$$aCalcium Carbonate: metabolism
000018198 650_2 $$2MeSH$$aChickens
000018198 650_2 $$2MeSH$$aCrystallization
000018198 650_2 $$2MeSH$$aEgg Shell: chemistry
000018198 650_2 $$2MeSH$$aEgg Shell: metabolism
000018198 650_2 $$2MeSH$$aEmulsions: chemistry
000018198 650_2 $$2MeSH$$aEmulsions: metabolism
000018198 650_2 $$2MeSH$$aMuramidase: chemistry
000018198 650_2 $$2MeSH$$aMuramidase: metabolism
000018198 650_2 $$2MeSH$$aOvalbumin: chemistry
000018198 650_2 $$2MeSH$$aOvalbumin: metabolism
000018198 650_7 $$00$$2NLM Chemicals$$aEmulsions
000018198 650_7 $$0471-34-1$$2NLM Chemicals$$aCalcium Carbonate
000018198 650_7 $$09006-59-1$$2NLM Chemicals$$aOvalbumin
000018198 650_7 $$0EC 3.2.1.17$$2NLM Chemicals$$aMuramidase
000018198 650_7 $$2WoSType$$aJ
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000018198 65017 $$0V:(DE-MLZ)GC-130-1$$2V:(DE-HGF)$$aHealth and Life$$x0
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000018198 7001_ $$0P:(DE-HGF)0$$aLeiterer, J.$$b1
000018198 7001_ $$0P:(DE-Juel1)VDB4339$$aPipich, V.$$b2$$uFZJ
000018198 7001_ $$0P:(DE-HGF)0$$aBarrea, R.$$b3
000018198 7001_ $$0P:(DE-HGF)0$$aEmmerling, F.$$b4
000018198 7001_ $$0P:(DE-HGF)0$$aTremel, W.$$b5
000018198 773__ $$0PERI:(DE-600)1472210-0$$a10.1021/ja202622g$$gVol. 133, p. 12642 - 12649$$p12642 - 12649$$q133<12642 - 12649$$tJournal of the American Chemical Society$$v133$$x0002-7863$$y2011
000018198 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170880
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