TY  - JOUR
AU  - Wu, Z.
AU  - Gogonea, V.
AU  - Lee, X.
AU  - May, R.P.
AU  - Pipich, V.
AU  - Wagner, M.A.
AU  - Undurti, A.
AU  - Tallant, T.C.
AU  - Baleanu-Gogonea, C.
AU  - Charlton, F.
AU  - Ioffe, A.
AU  - DiDonato, J.A.
AU  - Rye, K--A.
AU  - Hazen, S.L.
TI  - The low resolution structure of ApoA1 in spherical high density lipoprotein revealed by small angle neutron scattering
JO  - The journal of biological chemistry
VL  - 286
SN  - 0021-9258
CY  - Bethesda, Md.
PB  - Soc.
M1  - PreJuSER-18200
PY  - 2011
N1  - This work was supported, in whole or in part, by National Institutes of Health Grants P01 HL098055, P01 HL076491-055328, and P01 HL087018-02001. This work was also supported in part by a grant from the Foundation Leducq.
AB  - Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core. Three apoA1 chains are arranged within the low resolution structure of the protein component as one of three possible global architectures: (i) a helical dimer with a hairpin (HdHp), (ii) three hairpins (3Hp), or (iii) an integrated trimer (iT) in which the three apoA1 monomers mutually associate over a portion of the sHDL surface. Cross-linking and mass spectrometry analyses help to discriminate among the three molecular models and are most consistent with the HdHp overall architecture of apoA1 within sHDL.
KW  - Apolipoprotein A-I: chemistry
KW  - Humans
KW  - Lipoproteins, HDL: chemistry
KW  - Mass Spectrometry
KW  - Neutrons
KW  - Protein Multimerization
KW  - Scattering, Small Angle
KW  - Apolipoprotein A-I (NLM Chemicals)
KW  - Lipoproteins, HDL (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21292766
C2  - pmc:PMC3069452
UR  - <Go to ISI:>//WOS:000289077500063
DO  - DOI:10.1074/jbc.M110.209130
UR  - https://juser.fz-juelich.de/record/18200
ER  -