TY  - JOUR
AU  - Della Vecchia, Nicola Fyodor
AU  - Luchini, Alessandra
AU  - Napolitano, Alessandra
AU  - D’Errico, Gerardino
AU  - Vitiello, Giuseppe
AU  - Szekely, Noemi
AU  - d’Ischia, Marco
AU  - Paduano, Luigi
TI  - Tris Buffer Modulates Polydopamine Growth, Aggregation, and Paramagnetic Properties
JO  - Langmuir
VL  - 30
IS  - 32
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2014-06867
SP  - 9811 - 9818
PY  - 2014
AB  - Despite the growing technological interest of polydopamine (dopamine melanin)-based coatings for a broad variety of applications, the factors governing particle size, shape, and electronic properties of this bioinspired multifunctional material have remained little understood. Herein, we report a detailed characterization of polydopamine growth, particle morphology, and paramagnetic properties as a function of dopamine concentration and nature of the buffer (pH 8.5). Dynamic Light Scattering data revealed an increase in the hydrodynamic radii (Rh) of melanin particles with increasing dopamine concentration in all buffers examined, especially in phosphate buffer. Conversely, a marked inhibition of particle growth was apparent in Tris buffer, with Rh remaining as low as <100 nm during polymerization of 0.5 mM dopamine. Small angle neutron scattering data suggested formation of bidimensional structures in phosphate or bicarbonate buffers, while apparently three-dimensional fractal objects prevailed in Tris buffer. Finally, electron paramagnetic resonance spectra revealed a broader signal amplitude with a peculiar power saturation decay profile for polydopamine samples prepared in Tris buffer, denoting more homogeneous paramagnetic centers with respect to similar samples obtained in phosphate and bicarbonate buffers. Overall, these results disclose Tris buffer as an efficient modulator of polydopamine buildup and properties for the rational control and fine-tuning of melanin aggregate size, morphology, and free radical behavior.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000340701300022
DO  - DOI:10.1021/la501560z
UR  - https://juser.fz-juelich.de/record/183573
ER  -