TY  - JOUR
AU  - Bihr, Timo
AU  - Fenz, Susanne
AU  - Sackmann, Erich
AU  - Merkel, Rudolf
AU  - Seifert, Udo
AU  - Sengupta, Kheya
AU  - Smith, Ana-Sunčana
TI  - Association Rates of Membrane-Coupled Cell Adhesion Molecules
JO  - Biophysical journal
VL  - 107
IS  - 11
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - FZJ-2014-06965
SP  - L33 - L36
PY  - 2014
AB  - Thus far, understanding how the confined cellular environment affects the lifetime of bonds, as well as the extraction of complexation rates, has been a major challenge in studies of cell adhesion. Based on a theoretical description of the growth curves of adhesion domains, we present a new (to our knowledge) method to measure the association rate kon of ligand-receptor pairs incorporated into lipid membranes. As a proof of principle, we apply this method to several systems. We find that the kon for the interaction of biotin with neutravidin is larger than that for integrin binding to RGD or sialyl Lewisx to E-selectin. Furthermore, we find kon to be enhanced by membrane fluctuations that increase the probability for encounters between the binders. The opposite effect on kon could be attributed to the presence of repulsive polymers that mimic the glycocalyx, which points to two potential mechanisms for controlling the speed of protein complexation during the cell recognition process.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000345859500002
C6  - pmid:25468354
DO  - DOI:10.1016/j.bpj.2014.10.033
UR  - https://juser.fz-juelich.de/record/185538
ER  -