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@ARTICLE{Polovinkin:185976,
      author       = {Polovinkin, V. and Gushchin, I. and Sintsov, M. and Round,
                      E. and Balandin, T. and Chervakov, P. and Shevchenko, Vitaly
                      and Utrobin, P. and Popov, A. and Borshchevskiy, V. and
                      Mishin, A. and Kuklin, A. and Willbold, D. and Chupin, V.
                      and Popot, J-L and Gordeliy, V.},
      title        = {{H}igh-resolution structure of a membrane protein
                      transferred from amphipol to a lipidic mesophase.},
      journal      = {The journal of membrane biology},
      volume       = {247},
      number       = {9-10},
      issn         = {1432-1424},
      address      = {New York, NY},
      publisher    = {Springer},
      reportid     = {FZJ-2015-00085},
      pages        = {997 - 1004},
      year         = {2014},
      abstract     = {Amphipols (APols) have become important tools for the
                      stabilization, folding, and in vitro structural and
                      functional studies of membrane proteins (MPs). Direct
                      crystallization of MPs solubilized in APols would be of high
                      importance for structural biology. However, despite
                      considerable efforts, it is still not clear whether MP/APol
                      complexes can form well-ordered crystals suitable for X-ray
                      crystallography. In the present work, we show that an
                      APol-trapped MP can be crystallized in meso.
                      Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with
                      a lipidic mesophase, and crystallization was induced by
                      adding a precipitant. The crystals diffract beyond 2 Å. The
                      structure of BR was solved to 2 Å and found to be
                      indistinguishable from previous structures obtained after
                      transfer from detergent solutions. We suggest the proposed
                      protocol of in meso crystallization to be generally
                      applicable to APol-trapped MPs.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {452 - Structural Biology (POF2-452)},
      pid          = {G:(DE-HGF)POF2-452},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:25192977},
      UT           = {WOS:000343835500019},
      doi          = {10.1007/s00232-014-9700-x},
      url          = {https://juser.fz-juelich.de/record/185976},
}