000185993 001__ 185993
000185993 005__ 20220930130036.0
000185993 0247_ $$2doi$$a10.1371/journal.pone.0112873
000185993 0247_ $$2Handle$$a2128/8219
000185993 0247_ $$2WOS$$aWOS:000346907200012
000185993 0247_ $$2altmetric$$aaltmetric:4344698
000185993 0247_ $$2pmid$$apmid:25479443
000185993 037__ $$aFZJ-2015-00102
000185993 082__ $$a500
000185993 1001_ $$0P:(DE-Juel1)143908$$aShevchenko, Vitaly$$b0
000185993 245__ $$aCrystal Structure of Escherichia coli-Expressed Haloarcula marismortui Bacteriorhodopsin I in the Trimeric Form
000185993 260__ $$aLawrence, Kan.$$bPLoS$$c2014
000185993 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s185993
000185993 3367_ $$2DataCite$$aOutput Types/Journal article
000185993 3367_ $$00$$2EndNote$$aJournal Article
000185993 3367_ $$2BibTeX$$aARTICLE
000185993 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000185993 3367_ $$2DRIVER$$aarticle
000185993 520__ $$aBacteriorhodopsins are a large family of seven-helical transmembrane proteins that function as light-driven proton pumps. Here, we present the crystal structure of a new member of the family, Haloarcula marismortui bacteriorhodopsin I (HmBRI) D94N mutant, at the resolution of 2.5 Å. While the HmBRI retinal-binding pocket and proton donor site are similar to those of other archaeal proton pumps, its proton release region is extended and contains additional water molecules. The protein's fold is reinforced by three novel inter-helical hydrogen bonds, two of which result from double substitutions relative to Halobacterium salinarum bacteriorhodopsin and other similar proteins. Despite the expression in Escherichia coli and consequent absence of native lipids, the protein assembles as a trimer in crystals. The unique extended loop between the helices D and E of HmBRI makes contacts with the adjacent protomer and appears to stabilize the interface. Many lipidic hydrophobic tail groups are discernible in the membrane region, and their positions are similar to those of archaeal isoprenoid lipids in the crystals of other proton pumps, isolated from native or native-like sources. All these features might explain the HmBRI properties and establish the protein as a novel model for the microbial rhodopsin proton pumping studies.
000185993 536__ $$0G:(DE-HGF)POF2-452$$a452 - Structural Biology (POF2-452)$$cPOF2-452$$fPOF II$$x0
000185993 588__ $$aDataset connected to CrossRef, juser.fz-juelich.de
000185993 7001_ $$0P:(DE-Juel1)165798$$aGushchin, Ivan$$b1
000185993 7001_ $$0P:(DE-HGF)0$$aPolovinkin, V.$$b2
000185993 7001_ $$0P:(DE-HGF)0$$aRound, E.$$b3
000185993 7001_ $$0P:(DE-Juel1)144613$$aBorshchevskiy, Valentin$$b4
000185993 7001_ $$0P:(DE-HGF)0$$aUtrobin, P.$$b5
000185993 7001_ $$0P:(DE-HGF)0$$aPopov, A.$$b6
000185993 7001_ $$0P:(DE-Juel1)131949$$aBalandin, Taras$$b7
000185993 7001_ $$0P:(DE-Juel1)131957$$aBüldt, Georg$$b8
000185993 7001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b9$$eCorresponding Author
000185993 773__ $$0PERI:(DE-600)2267670-3$$a10.1371/journal.pone.0112873$$gVol. 9, no. 12, p. e112873 -$$n12$$pe112873$$tPLoS one$$v9$$x1932-6203$$y2014
000185993 8564_ $$uhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0112873
000185993 8564_ $$uhttps://juser.fz-juelich.de/record/185993/files/FZJ-2015-00102.pdf$$yOpenAccess
000185993 8564_ $$uhttps://juser.fz-juelich.de/record/185993/files/FZJ-2015-00102.jpg?subformat=icon-144$$xicon-144$$yOpenAccess
000185993 8564_ $$uhttps://juser.fz-juelich.de/record/185993/files/FZJ-2015-00102.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000185993 8564_ $$uhttps://juser.fz-juelich.de/record/185993/files/FZJ-2015-00102.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000185993 8767_ $$92015-01-05$$d2015-01-09$$eAPC$$jDeposit$$lDeposit: PLoS$$zUSD 1.350,-
000185993 909CO $$ooai:juser.fz-juelich.de:185993$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire$$pdnbdelivery
000185993 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)143908$$aForschungszentrum Jülich GmbH$$b0$$kFZJ
000185993 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165798$$aForschungszentrum Jülich GmbH$$b1$$kFZJ
000185993 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131949$$aForschungszentrum Jülich GmbH$$b7$$kFZJ
000185993 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131957$$aForschungszentrum Jülich GmbH$$b8$$kFZJ
000185993 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131964$$aForschungszentrum Jülich GmbH$$b9$$kFZJ
000185993 9132_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0
000185993 9131_ $$0G:(DE-HGF)POF2-452$$1G:(DE-HGF)POF2-450$$2G:(DE-HGF)POF2-400$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$aDE-HGF$$bSchlüsseltechnologien$$lBioSoft$$vStructural Biology$$x0
000185993 9141_ $$y2014
000185993 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000185993 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000185993 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000185993 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000185993 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000185993 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000185993 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000185993 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000185993 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000185993 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF <  5
000185993 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000185993 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000185993 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000185993 920__ $$lyes
000185993 9201_ $$0I:(DE-Juel1)ICS-6-20110106$$kICS-6$$lStrukturbiochemie $$x0
000185993 9801_ $$aFullTexts
000185993 980__ $$ajournal
000185993 980__ $$aVDB
000185993 980__ $$aUNRESTRICTED
000185993 980__ $$aFullTexts
000185993 980__ $$aI:(DE-Juel1)ICS-6-20110106
000185993 980__ $$aAPC
000185993 981__ $$aI:(DE-Juel1)IBI-7-20200312