TY  - JOUR
AU  - Galkin, Vitold E.
AU  - Orlova, Albina
AU  - Vos, Matthijn R.
AU  - Schröder, Gunnar
AU  - Egelman, Edward H.
TI  - Near-Atomic Resolution for One State of F-Actin
JO  - Structure
VL  - 23
IS  - 1
SN  - 0969-2126
CY  - London [u.a.]
PB  - Elsevier Science
M1  - FZJ-2015-00729
SP  - 173 - 182
PY  - 2015
AB  - Actin functions as a helical polymer, F-actin, but attempts to build an atomic model for this filament have been hampered by the fact that the filament cannot be crystallized and by structural heterogeneity. We have used a direct electron detector, cryo-electron microscopy, and the forces imposed on actin filaments in thin films to reconstruct one state of the filament at 4.7 Å resolution, which allows for building a reliable pseudo-atomic model of F-actin. We also report a different state of the filament where actin protomers adopt a conformation observed in the crystal structure of the G-actin-profilin complex with an open ATP-binding cleft. Comparison of the two structural states provides insights into ATP-hydrolysis and filament dynamics. The atomic model provides a framework for understanding why every buried residue in actin has been under intense selective pressure.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000347469500021
C6  - pmid:25533486
DO  - DOI:10.1016/j.str.2014.11.006
UR  - https://juser.fz-juelich.de/record/186656
ER  -