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@ARTICLE{Galkin:186656,
      author       = {Galkin, Vitold E. and Orlova, Albina and Vos, Matthijn R.
                      and Schröder, Gunnar and Egelman, Edward H.},
      title        = {{N}ear-{A}tomic {R}esolution for {O}ne {S}tate of
                      {F}-{A}ctin},
      journal      = {Structure},
      volume       = {23},
      number       = {1},
      issn         = {0969-2126},
      address      = {London [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2015-00729},
      pages        = {173 - 182},
      year         = {2015},
      abstract     = {Actin functions as a helical polymer, F-actin, but attempts
                      to build an atomic model for this filament have been
                      hampered by the fact that the filament cannot be
                      crystallized and by structural heterogeneity. We have used a
                      direct electron detector, cryo-electron microscopy, and the
                      forces imposed on actin filaments in thin films to
                      reconstruct one state of the filament at 4.7 Å resolution,
                      which allows for building a reliable pseudo-atomic model of
                      F-actin. We also report a different state of the filament
                      where actin protomers adopt a conformation observed in the
                      crystal structure of the G-actin-profilin complex with an
                      open ATP-binding cleft. Comparison of the two structural
                      states provides insights into ATP-hydrolysis and filament
                      dynamics. The atomic model provides a framework for
                      understanding why every buried residue in actin has been
                      under intense selective pressure.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000347469500021},
      pubmed       = {pmid:25533486},
      doi          = {10.1016/j.str.2014.11.006},
      url          = {https://juser.fz-juelich.de/record/186656},
}