guest ::
| Home > Publications database > Near-Atomic Resolution for One State of F-Actin > print |
| 001 | 186656 | ||
| 005 | 20210129214931.0 | ||
| 024 | 7 | _ | |a 10.1016/j.str.2014.11.006 |2 doi |
| 024 | 7 | _ | |a 0969-2126 |2 ISSN |
| 024 | 7 | _ | |a 1878-4186 |2 ISSN |
| 024 | 7 | _ | |a WOS:000347469500021 |2 WOS |
| 024 | 7 | _ | |a altmetric:3010138 |2 altmetric |
| 024 | 7 | _ | |a pmid:25533486 |2 pmid |
| 037 | _ | _ | |a FZJ-2015-00729 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Galkin, Vitold E. |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Near-Atomic Resolution for One State of F-Actin |
| 260 | _ | _ | |a London [u.a.] |c 2015 |b Elsevier Science |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1421852668_13878 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 520 | _ | _ | |a Actin functions as a helical polymer, F-actin, but attempts to build an atomic model for this filament have been hampered by the fact that the filament cannot be crystallized and by structural heterogeneity. We have used a direct electron detector, cryo-electron microscopy, and the forces imposed on actin filaments in thin films to reconstruct one state of the filament at 4.7 Å resolution, which allows for building a reliable pseudo-atomic model of F-actin. We also report a different state of the filament where actin protomers adopt a conformation observed in the crystal structure of the G-actin-profilin complex with an open ATP-binding cleft. Comparison of the two structural states provides insights into ATP-hydrolysis and filament dynamics. The atomic model provides a framework for understanding why every buried residue in actin has been under intense selective pressure. |
| 536 | _ | _ | |a 551 - Functional Macromolecules and Complexes (POF3-551) |0 G:(DE-HGF)POF3-551 |c POF3-551 |x 0 |f POF III |
| 588 | _ | _ | |a Dataset connected to CrossRef, juser.fz-juelich.de |
| 700 | 1 | _ | |a Orlova, Albina |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Vos, Matthijn R. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Schröder, Gunnar |0 P:(DE-Juel1)132018 |b 3 |u fzj |
| 700 | 1 | _ | |a Egelman, Edward H. |0 P:(DE-HGF)0 |b 4 |e Corresponding Author |
| 773 | _ | _ | |a 10.1016/j.str.2014.11.006 |g Vol. 23, no. 1, p. 173 - 182 |0 PERI:(DE-600)2031189-8 |n 1 |p 173 - 182 |t Structure |v 23 |y 2015 |x 0969-2126 |
| 856 | 4 | _ | |u http://www.sciencedirect.com/science/article/pii/S0969212614003724 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/186656/files/FZJ-2015-00729.pdf |y Restricted |
| 909 | C | O | |o oai:juser.fz-juelich.de:186656 |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)132018 |
| 913 | 0 | _ | |a DE-HGF |b Schlüsseltechnologien |l BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |1 G:(DE-HGF)POF2-450 |0 G:(DE-HGF)POF2-452 |2 G:(DE-HGF)POF2-400 |v Structural Biology |x 0 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-551 |2 G:(DE-HGF)POF3-500 |v Functional Macromolecules and Complexes |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2015 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)ICS-6-20110106 |k ICS-6 |l Strukturbiochemie |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|