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@ARTICLE{Liebl:187350,
      author       = {Liebl, Wolfgang and Angelov, Angel and Juergensen, Julia
                      and Chow, Jennifer and Loeschcke, Anita and Drepper, Thomas
                      and Classen, Thomas and Ehrenreich, Armin and Streit,
                      Wolfgang R. and Jaeger, Karl-Erich and Pietruszka, Jörg},
      title        = {{A}lternative hosts for functional (meta)genome analysis},
      journal      = {Applied microbiology and biotechnology},
      volume       = {98},
      number       = {19},
      issn         = {1432-0614},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {FZJ-2015-01021},
      pages        = {8099 - 8109},
      year         = {2014},
      abstract     = {Microorganisms are ubiquitous on earth, often forming
                      complex microbial communities in numerous different
                      habitats. Most of these organisms cannot be readily
                      cultivated in the laboratory using standard media and growth
                      conditions. However, it is possible to gain access to the
                      vast genetic, enzymatic, and metabolic diversity present in
                      these microbial communities using cultivation-independent
                      approaches such as sequence- or function-based metagenomics.
                      Function-based analysis is dependent on heterologous
                      expression of metagenomic libraries in a genetically
                      amenable cloning and expression host. To date, Escherichia
                      coli is used in most cases; however, this has the drawback
                      that many genes from heterologous genomes and complex
                      metagenomes are expressed in E. coli either at very low
                      levels or not at all. This review emphasizes the importance
                      of establishing alternative microbial expression systems
                      consisting of different genera and species as well as
                      customized strains and vectors optimized for heterologous
                      expression of membrane proteins, multigene clusters encoding
                      protein complexes or entire metabolic pathways. The use of
                      alternative host-vector systems will complement current
                      metagenomic screening efforts and expand the yield of novel
                      biocatalysts, metabolic pathways, and useful metabolites to
                      be identified from environmental samples.},
      cin          = {IBOC / IBG-1 / IMET},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118 /
                      I:(DE-Juel1)IMET-20090612},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000342072500005},
      pubmed       = {pmid:25091044},
      doi          = {10.1007/s00253-014-5961-7},
      url          = {https://juser.fz-juelich.de/record/187350},
}