TY  - JOUR
AU  - Schellenberg, Anne
AU  - Joussen, Sylvia
AU  - Moser, Kristin
AU  - Hampe, Nico
AU  - Hersch, Nils
AU  - Hemeda, Hatim
AU  - Schnitker, Jan
AU  - Denecke, Bernd
AU  - Lin, Qiong
AU  - Pallua, Norbert
AU  - Zenke, Martin
AU  - Merkel, Rudolf
AU  - Hoffmann, Bernd
AU  - Wagner, Wolfgang
TI  - Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells
JO  - Biomaterials
VL  - 35
IS  - 24
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2015-01259
SP  - 6351-6358
PY  - 2014
AB  - Matrix elasticity guides differentiation of mesenchymal stem cells (MSCs) but it is unclear if these effects are only transient – while the cells reside on the substrate – or if they reflect persistent lineage commitment. In this study, MSCs were continuously culture-expanded in parallel either on tissue culture plastic (TCP) or on polydimethylsiloxane (PDMS) gels of different elasticity to compare impact on replicative senescence, in vitro differentiation, gene expression, and DNA methylation (DNAm) profiles. The maximal number of cumulative population doublings was not affected by matrix elasticity. Differentiation towards adipogenic and osteogenic lineage was increased on soft and rigid biomaterials, respectively – but this propensity was no more evident if cells were transferred to TCP. Global gene expression profiles and DNAm profiles revealed relatively few differences in MSCs cultured on soft or rigid matrices. Furthermore, only moderate DNAm changes were observed upon culture on very soft hydrogels of human platelet lysate. Our results support the notion that matrix elasticity influences cellular behavior while the cells reside on the substrate, but it does not have major impact on cell-intrinsic lineage determination, replicative senescence or DNAm patterns.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000338804500020
C6  - pmid:24824582
DO  - DOI:10.1016/j.biomaterials.2014.04.079
UR  - https://juser.fz-juelich.de/record/187633
ER  -