000188031 001__ 188031
000188031 005__ 20210129215123.0
000188031 0247_ $$2Handle$$a2128/15463
000188031 037__ $$aFZJ-2015-01516
000188031 041__ $$aEnglish
000188031 1001_ $$0P:(DE-Juel1)133346$$aSchmitz, Sabine$$b0$$eCorresponding Author$$ufzj
000188031 1112_ $$a11th International Symposium on Chromosomal Aberrations$$cRhodes$$d2014-09-12 - 2014-09-14$$gISCA$$wGreek
000188031 245__ $$aChromosome aberrations induced by the Auger emitter I-125
000188031 260__ $$c2014
000188031 3367_ $$033$$2EndNote$$aConference Paper
000188031 3367_ $$2DataCite$$aOther
000188031 3367_ $$2BibTeX$$aINPROCEEDINGS
000188031 3367_ $$2DRIVER$$aconferenceObject
000188031 3367_ $$2ORCID$$aLECTURE_SPEECH
000188031 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1507191672_26345$$xInvited
000188031 520__ $$aIntroduction: DNA-associated Auger-electron emitters (AEE) induce cellular damage leading to high-LET type cell survival curves and possess enhanced relative biological effectiveness. DNA dsb induced by Iodine-125-deoxyuridine (I-125-UdR) decays are claimed to be very complex, thus efficiently leading to cell transformation, gene mutation and induction of chromatid aberrations. To elucidate the assumed genotoxic potential of DNA-associated AEE, chromosomal/chromatid aberrations were analyzed in I-125-UdR-exposed human peripheral blood lymphocytes (PBL).Methods: PBL were isolated from whole blood and stimulated with chromosome medium containing phytohaemagglutinin (PHA). After 24 h cultures were labeled with I-125-UdR for 18 h (0.25-4.5 kBq/ml) during the S-phase of the cell cycle. After removal of radioactive medium and washing steps, cells were re-cultured in stimulation medium for further 24 h. Colcemid was added 5.5 h before harvest of cells followed by fixation for aberrations at 71.5 h post-stimulation. All slides were stained with 10 % Giemsa, and 100 metaphases were analyzed microscopically for each dose point.Results: After 18 h labeling with I-125-UdR the cell cycle distribution is severely disturbed. Furthermore, 40% of PBL are fully labelled and 20% show a moderate uptake of I-125-UdR. I-125-UdR primarily induces chromatid-type aberrations. PBL reveal a very broad dose-dependent response spectrum: equal numbers of cells have either no aberration, or display a moderate aberration level (1-9 aberrations). Few cells exhibit a high aberration score (> 10 aberrations). A dose-dependent increase of aberrations is measured in the range of 0.2 to 2 Gy, followed by a plateau between 2 and 4.5 Gy. The data indicate that even the lowest dose of 0.2 Gy leads to significant damage in PBL and to a 4.5-fold increase of aberrations compared to the controls. Furthermore, a dose-dependent increase of cell death is observed.Conclusions: I-125-UdR has a very strong genotoxic capacity in human PBL even at very low doses of about 0.2 Gy. Efficiently labeled cells displaying a prolonged cell cycle compared to moderate labeled cells, and cell death contribute substantially to the desynchronisation of the cell cycle. In summary, it can be concluded that every fourth intracellular I-125 decay give rise to a single chromosome aberration.
000188031 536__ $$0G:(DE-HGF)POF3-899$$a899 - ohne Topic (POF3-899)$$cPOF3-899$$fPOF III$$x0
000188031 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000188031 7001_ $$0P:(DE-Juel1)133339$$aOskamp, Dominik$$b1$$ufzj
000188031 7001_ $$0P:(DE-Juel1)133341$$aPomplun, Ekkehard$$b2$$ufzj
000188031 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b3$$ufzj
000188031 773__ $$y2014
000188031 8564_ $$uhttps://juser.fz-juelich.de/record/188031/files/Invited%20Talk%20Schmitz%20ISCA%202014.pdf$$yOpenAccess
000188031 8564_ $$uhttps://juser.fz-juelich.de/record/188031/files/Invited%20Talk%20Schmitz%20ISCA%202014.gif?subformat=icon$$xicon$$yOpenAccess
000188031 8564_ $$uhttps://juser.fz-juelich.de/record/188031/files/Invited%20Talk%20Schmitz%20ISCA%202014.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000188031 8564_ $$uhttps://juser.fz-juelich.de/record/188031/files/Invited%20Talk%20Schmitz%20ISCA%202014.jpg?subformat=icon-700$$xicon-700$$yOpenAccess
000188031 8564_ $$uhttps://juser.fz-juelich.de/record/188031/files/Invited%20Talk%20Schmitz%20ISCA%202014.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000188031 909CO $$ooai:juser.fz-juelich.de:188031$$pdriver$$pVDB$$popen_access$$popenaire
000188031 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133346$$aForschungszentrum Jülich GmbH$$b0$$kFZJ
000188031 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133339$$aForschungszentrum Jülich GmbH$$b1$$kFZJ
000188031 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133341$$aForschungszentrum Jülich GmbH$$b2$$kFZJ
000188031 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133469$$aForschungszentrum Jülich GmbH$$b3$$kFZJ
000188031 9131_ $$0G:(DE-HGF)POF3-899$$1G:(DE-HGF)POF3-890$$2G:(DE-HGF)POF3-800$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000188031 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000188031 920__ $$lyes
000188031 9201_ $$0I:(DE-Juel1)S-US-20090406$$kS-US$$lSicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie$$x0
000188031 980__ $$aconf
000188031 980__ $$aVDB
000188031 980__ $$aUNRESTRICTED
000188031 980__ $$aI:(DE-Juel1)S-US-20090406
000188031 9801_ $$aFullTexts