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@ARTICLE{Coates:188456,
author = {Coates, Leighton and Tomanicek, Stephen and Schrader,
Tobias E. and Weiss, Kevin L. and Ng, Joseph D. and
Jüttner, Philipp and Ostermann, Andreas},
title = {{C}ryogenic neutron protein crystallography: routine
methods and potential benefits},
journal = {Journal of applied crystallography},
volume = {47},
number = {4},
issn = {1600-5767},
address = {Copenhagen},
publisher = {Munksgaard},
reportid = {FZJ-2015-01832},
pages = {1431 - 1434},
year = {2014},
abstract = {The use of cryocooling in neutron diffraction has been
hampered by several technical challenges, such as the need
for specialized equipment and techniques. This article
reports the recent development and deployment of equipment
and strategies that allow routine neutron data collection on
cryocooled crystals using off-the-shelf components. This
system has several advantages compared to a closed displex
cooling system, such as fast cooling coupled with easier
crystal mounting and centering. The ability to routinely
collect cryogenic neutron data for analysis will
significantly broaden the range of scientific questions that
can be examined by neutron protein crystallography.
Cryogenic neutron data collection for macromolecules has
recently become available at the new Biological
Diffractometer BIODIFF at the FRM II and the Macromolecular
Diffractometer (MaNDi) at the Spallation Neutron Source, Oak
Ridge National Laboratory. To evaluate the benefits of a
cryocooled neutron structure, a full neutron data set was
collected on the BIODIFF instrument on a Toho-1
[beta]-lactamase structure at 100 K.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {54G - JCNS (POF2-54G24)},
pid = {G:(DE-HGF)POF2-54G24},
experiment = {EXP:(DE-MLZ)BIODIFF-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000340362000030},
doi = {10.1107/S1600576714010772},
url = {https://juser.fz-juelich.de/record/188456},
}