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@ARTICLE{Yokoyama:188458,
author = {Yokoyama, Takeshi and Ostermann, Andreas and Mizuguchi,
Mineyuki and Niimura, Nobuo and Schrader, Tobias E. and
Tanaka, Ichiro},
title = {{C}rystallization and preliminary neutron diffraction
experiment of human farnesyl pyrophosphate synthase
complexed with risedronate},
journal = {Acta crystallographica / F},
volume = {70},
issn = {1744-3091},
address = {Oxford [u.a.]},
publisher = {Blackwell},
reportid = {FZJ-2015-01834},
pages = {470},
year = {2014},
abstract = {Nitrogen-containing bisphosphonates (N-BPs), such as
risedronate and zoledronate, are currently used as a
clinical drug for bone-resorption diseases and are potent
inhibitors of farnesyl pyrophosphate synthase (FPPS). X-ray
crystallographic analyses of FPPS with N-BPs have revealed
that N-BPs bind to FPPS with three magnesium ions and
several water molecules. To understand the structural
characteristics of N-BPs bound to FPPS, including H atoms
and hydration by water, neutron diffraction studies were
initiated using BIODIFF at the Heinz Maier-Leibnitz Zentrum
(MLZ). FPPS-risedronate complex crystals of approximate
dimensions 2.8 × 2.5 × 1.5 mm (~3.5 mm3) were obtained by
repeated macro-seeding. Monochromatic neutron diffraction
data were collected to 2.4 Å resolution with $98.4\%$
overall completeness. Here, the first successful neutron
data collection from FPPS in complex with N-BPs is
reported.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {530},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {54G - JCNS (POF2-54G24)},
pid = {G:(DE-HGF)POF2-54G24},
experiment = {EXP:(DE-MLZ)BIODIFF-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000333757800014},
pubmed = {pmid:24699741},
doi = {10.1107/S2053230X14004087},
url = {https://juser.fz-juelich.de/record/188458},
}