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@ARTICLE{Schmidt:188597,
author = {Schmidt, Daniel and Monzel, Cornelia and Bihr, Timo and
Merkel, Rudolf and Seifert, Udo and Sengupta, Kheya and
Smith, Ana-Sunčana},
title = {{S}ignature of a {N}onharmonic {P}otential as {R}evealed
from a {C}onsistent {S}hape and {F}luctuation {A}nalysis of
an {A}dherent {M}embrane},
journal = {Physical review / X},
volume = {4},
number = {2},
issn = {2160-3308},
address = {College Park, Md.},
publisher = {APS},
reportid = {FZJ-2015-01940},
pages = {021023},
year = {2014},
abstract = {The interaction of fluid membranes with a scaffold, which
can be a planar surface or a more complex structure, is
intrinsic to a number of systems from artificial supported
bilayers and vesicles to cellular membranes. In principle,
these interactions can be either discrete and protein
mediated, or continuous. In the latter case, they emerge
from ubiquitous intrinsic surface interaction potentials as
well as nature-designed steric contributions of the
fluctuating membrane or from the polymers of the glycocalyx.
Despite the fact that these nonspecific potentials are
omnipresent, their description has been a major challenge
from experimental and theoretical points of view. Here, we
show that a full understanding of the implications of the
continuous interactions can be achieved only by expanding
the standard superposition models commonly used to treat
these types of systems, beyond the usual harmonic level of
description. Supported by this expanded theoretical
framework, we present three independent, yet mutually
consistent, experimental approaches to measure the
interaction potential strength and the membrane tension.
Upon explicitly taking into account the nature of shot noise
as well as the nature of finite experimental resolution,
excellent agreement with the augmented theory is obtained,
which finally provides a coherent view of the behavior of
the membrane in the vicinity of a scaffold.},
cin = {ICS-7},
ddc = {530},
cid = {I:(DE-Juel1)ICS-7-20110106},
pnm = {453 - Physics of the Cell (POF2-453)},
pid = {G:(DE-HGF)POF2-453},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000336121500002},
doi = {10.1103/PhysRevX.4.021023},
url = {https://juser.fz-juelich.de/record/188597},
}