TY  - CONF
AU  - Unverricht, Marcus
AU  - Giesen
AU  - Pomplun, Ekkehard
AU  - Kriehuber, Ralf
TI  - Comparative analysis of gene expression data after exposure to I-123-iododeoxyuridine, γ-rays and α-particles
M1  - FZJ-2015-02036
PY  - 2014
AB  - Gene expression analysis was carried out in Jurkat cells in order to identify robust candidate genes showing significant gene expression alterations allowing the discrimination of radiation qualities.Equi-effect radiation doses, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined with regard to micronucleus formation, γ-H2AX foci signal intensity and apoptosis induction after γ-irradiation, α-irradiation and exposure to the Auger electron emitter I-123-iododeoxyuridine (123IdU). Microarray gene expression analysis was performed after exposure to equi-effect radiation doses of all three radiation qualities. Candidate genes for the discrimination of radiation quality have to show a significant expression change after exposure to a specific radiation quality and display no altered gene regulation or a conversely regulation in response to exposure to the other radiation qualities investigated. Gene expression of selected candidate genes was validated via qRT-PCR. Biological processes and pathways of significantly regulated genes were subsequently analyzed.At equi-effect doses the results of the gene expression analysis showed that 359, 598 and 1339 genes are significantly regulated after exposure to 123IdU, α-particles and γ-rays, respectively. Only 4, 1 and 1 gene(s) are identified allowing a reliable discrimination between γ- vs. 123IdU-exposure, γ- vs. α-radiation and α- vs. 123IdU-exposure, respectively.γ-rays induce pronounced alterations in gene expression in Jurkat cells when compared to 123IdU and α-particles at equi-effect radiation doses. Gene expression analysis in Jurkat cells might suggest that the discrimination of different radiation qualities by means of gene expression is possible.
T2  - 17th Annual Meeting of the German Society for Biological Radiation Research
CY  - 29 Sep 2014 - 1 Oct 2014, Tübingen (Germany)
Y2  - 29 Sep 2014 - 1 Oct 2014
M2  - Tübingen, Germany
LB  - PUB:(DE-HGF)24
UR  - https://juser.fz-juelich.de/record/188712
ER  -