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@INPROCEEDINGS{Unverricht:188712,
      author       = {Unverricht, Marcus and Giesen and Pomplun, Ekkehard and
                      Kriehuber, Ralf},
      title        = {{C}omparative analysis of gene expression data after
                      exposure to {I}-123-iododeoxyuridine, γ-rays and
                      α-particles},
      reportid     = {FZJ-2015-02036},
      year         = {2014},
      abstract     = {Gene expression analysis was carried out in Jurkat cells in
                      order to identify robust candidate genes showing significant
                      gene expression alterations allowing the discrimination of
                      radiation qualities.Equi-effect radiation doses, i.e.
                      radiation doses and exposure conditions causing the same
                      biological effect level, were determined with regard to
                      micronucleus formation, γ-H2AX foci signal intensity and
                      apoptosis induction after γ-irradiation, α-irradiation and
                      exposure to the Auger electron emitter
                      I-123-iododeoxyuridine (123IdU). Microarray gene expression
                      analysis was performed after exposure to equi-effect
                      radiation doses of all three radiation qualities. Candidate
                      genes for the discrimination of radiation quality have to
                      show a significant expression change after exposure to a
                      specific radiation quality and display no altered gene
                      regulation or a conversely regulation in response to
                      exposure to the other radiation qualities investigated. Gene
                      expression of selected candidate genes was validated via
                      qRT-PCR. Biological processes and pathways of significantly
                      regulated genes were subsequently analyzed.At equi-effect
                      doses the results of the gene expression analysis showed
                      that 359, 598 and 1339 genes are significantly regulated
                      after exposure to 123IdU, α-particles and γ-rays,
                      respectively. Only 4, 1 and 1 gene(s) are identified
                      allowing a reliable discrimination between γ- vs.
                      123IdU-exposure, γ- vs. α-radiation and α- vs.
                      123IdU-exposure, respectively.γ-rays induce pronounced
                      alterations in gene expression in Jurkat cells when compared
                      to 123IdU and α-particles at equi-effect radiation doses.
                      Gene expression analysis in Jurkat cells might suggest that
                      the discrimination of different radiation qualities by means
                      of gene expression is possible.},
      month         = {Sep},
      date          = {2014-09-29},
      organization  = {17th Annual Meeting of the German
                       Society for Biological Radiation
                       Research, Tübingen (Germany), 29 Sep
                       2014 - 1 Oct 2014},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/188712},
}