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@ARTICLE{Albers:188772,
      author       = {Albers, Jonas and Toma, Koji and Offenhäusser, Andreas},
      title        = {{E}ngineering connectivity by multiscale micropatterning of
                      individual populations of neurons},
      journal      = {Biotechnology journal},
      volume       = {10},
      number       = {2},
      issn         = {1860-6768},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2015-02091},
      pages        = {332 - 338},
      year         = {2015},
      abstract     = {Functional networks are the basis of information processing
                      in the central nervous system. Essential for their formation
                      are guided neuronal growth as well as controlled
                      connectivity and information flow. The basis of neuronal
                      development is generated by guiding cues and geometric
                      constraints. To investigate the neuronal growth and
                      connectivity of adjacent neuronal networks, two-dimensional
                      protein patterns were created. A mixture of poly-L-lysine
                      and laminin was transferred onto a silanized glass surface
                      by microcontact printing. The structures were populated with
                      dissociated primary cortical embryonic rat neurons.
                      Triangular structures with diverse opening angles, height,
                      and design were chosen as two-dimensional structures to
                      allow network formation with constricted gateways. Neuronal
                      development was observed by immunohistochemistry to pursue
                      the influence of the chosen structures on the neuronal
                      outgrowth. Neurons were stained for MAP2, while
                      poly-L-lysine was FITC labeled. With this study we present
                      an easy-to-use technique to engineer two-dimensional
                      networks in vitro with defined gateways. The presented
                      micropatterning method is used to generate daisy-chained
                      neuronal networks with predefined connectivity. Signal
                      propagation among geometrically constrained networks can
                      easily be monitored by calcium-sensitive dyes, providing
                      insights into network communication in vitro.},
      cin          = {ICS-8 / PGI-8 / JARA-FIT},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)PGI-8-20110106 /
                      $I:(DE-82)080009_20140620$},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000350104700014},
      pubmed       = {pmid:25512037},
      doi          = {10.1002/biot.201400609},
      url          = {https://juser.fz-juelich.de/record/188772},
}