TY  - JOUR
AU  - Schmittgen, S.
AU  - Metzner, R.
AU  - Van Dusschoten, D.
AU  - Jansen, M.
AU  - Fiorani, F.
AU  - Jahnke, S.
AU  - Rascher, U.
AU  - Schurr, U.
TI  - Magnetic resonance imaging of sugar beet taproots in soil reveals growth reduction and morphological changes during foliar Cercospora beticola infestation
JO  - The journal of experimental botany
VL  - 66
IS  - 18
SN  - 1460-2431
CY  - Oxford
PB  - Univ. Press
M1  - FZJ-2015-02345
SP  - 5543-5553
PY  - 2015
AB  - Cercospora leaf spot (CLS) infection can cause severe yield loss in sugar beet. Introduction of Cercospora-resistant varieties in breeding programmes is important for plant protection to reduce both fungicide applications and the risk of the development of fungal resistance. However, in vivo monitoring of the sugar-containing taproots at early stages of foliar symptoms and the characterization of the temporal development of disease progression has proven difficult. Non-invasive magnetic resonance imaging (MRI) measurements were conducted to quantify taproot development of genotypes with high (HS) and low (LS) levels of susceptibility after foliar Cercospora inoculation. Fourteen days post-inoculation (dpi) the ratio of infected leaf area was still low (~7%) in both the HS and LS genotypes. However, during this period, the volumetric growth of the taproot had already started to decrease. Additionally, inoculated plants showed a reduction of the increase in width of inner cambial rings while the width of outer rings increased slightly compared with non-inoculated plants. This response partly compensated for the reduced development of inner rings that had a vascular connection with Cercospora-inoculated leaves. Hence, alterations in taproot anatomical features such as volume and cambial ring development can be non-invasively detected already at 14 dpi, providing information on the early impact of the infection on whole-plant performance. All these findings show that MRI is a suitable tool to identify promising candidate parent lines with improved resistance to Cercospora, for example with comparatively lower taproot growth reduction at early stages of canopy infection, for future introduction into breeing programmes. 
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000361208000014
C6  - pmid:25873673
DO  - DOI:10.1093/jxb/erv109
UR  - https://juser.fz-juelich.de/record/189145
ER  -