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@ARTICLE{Qi:189196,
      author       = {Qi, Guanxiao and Radnikow, Gabriele and Feldmeyer, Dirk},
      title        = {{E}lectrophysiological and {M}orphological
                      {C}haracterization of {N}euronal {M}icrocircuits in {A}cute
                      {B}rain {S}lices {U}sing {P}aired {P}atch-{C}lamp
                      {R}ecordings},
      journal      = {Journal of visualized experiments},
      volume       = {},
      number       = {95},
      issn         = {1940-087X},
      address      = {[S.l.]},
      publisher    = {JoVE},
      reportid     = {FZJ-2015-02387},
      pages        = {52358},
      year         = {2015},
      abstract     = {The combination of patch clamp recordings from two (or
                      more) synaptically coupled neurons (paired recordings) in
                      acute brain slice preparations with simultaneous
                      intracellular biocytin filling allows a correlated analysis
                      of their structural and functional properties. With this
                      method it is possible to identify and characterize both pre-
                      and postsynaptic neurons by their morphology and
                      electrophysiological response pattern. Paired recordings
                      allow studying the connectivity patterns between these
                      neurons as well as the properties of both chemical and
                      electrical synaptic transmission. Here, we give a
                      step-by-step description of the procedures required to
                      obtain reliable paired recordings together with an optimal
                      recovery of the neuron morphology. We will describe how
                      pairs of neurons connected via chemical synapses or gap
                      junctions are identified in brain slice preparations. We
                      will outline how neurons are reconstructed to obtain their
                      3D morphology of the dendritic and axonal domain and how
                      synaptic contacts are identified and localized. We will also
                      discuss the caveats and limitations of the paired recording
                      technique, in particular those associated with dendritic and
                      axonal truncations during the preparation of brain slices
                      because these strongly affect connectivity estimates.
                      However, because of the versatility of the paired recording
                      approach it will remain a valuable tool in characterizing
                      different aspects of synaptic transmission at identified
                      neuronal microcircuits in the brain.},
      cin          = {INM-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)INM-2-20090406},
      pnm          = {571 - Connectivity and Activity (POF3-571)},
      pid          = {G:(DE-HGF)POF3-571},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000361532900060},
      pubmed       = {pmid:25650985},
      doi          = {10.3791/52358},
      url          = {https://juser.fz-juelich.de/record/189196},
}